Distribution of secretoneurin, a peptide derived from secretogranin II, in rat brain

An immunocytochemical and radioimmunological study

J. Marksteiner, R. Kirchmair, S. K. Mahata, M. Mahata, R. Fischer-Colbrie, R. Hogue-Angeletti, A. Saria, H. Winkler

Research output: Contribution to journalArticle

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Abstract

The distribution of secretoneurin, a peptide derived from its precursor secretogranin II by proteolytic processing, was studied in the central nervous system of the rat by immunocytochemistry and radioimmunoassay and compared to the distribution of secretogranin II messenger RNA by using in situ hybridization. With a specific antiserum a distinct staining of fibers and to a lesser extent also of perikarya was observed throughout the central nervous system. A high density of immunoreactive fibers and terminals was found in several brain areas, i.e. the lateral septum, the medial parts of the amygdala, some medial thalamic nuclei, the hypothalamus, habenula, nucleus interpeduncularis, locus coeruleus, nucleus tractus solitarii, the substantiae gelatinosae of the caudal trigeminal nucleus and of the spinal cord. The quantitative distribution as measured by a radioimmunoassay agreed well with the varying densities of immunoreactivity found by immunocytochemistry. The highest concentrations of this peptide were present in the hypothalamus, in particular, in the median eminence and are comparable to those of the most highly concentrated neuropeptides. The distribution of immunopositive perikarya corresponded well with that of secretogranin II messenger RNA obtained by in situ hybridization. The pattern of secretoneurin expression in rat brain was widespread and unique, partially overlapping with established chemical transmitters and neuropeptides. The functional significance of this new brain peptide remains to be established.

Original languageEnglish (US)
Pages (from-to)923-944
Number of pages22
JournalNeuroscience
Volume54
Issue number4
DOIs
StatePublished - 1993

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Secretogranin II
Neuropeptides
Peptides
Hypothalamus
Radioimmunoassay
In Situ Hybridization
Trigeminal Caudal Nucleus
Brain
Central Nervous System
Immunohistochemistry
Habenula
Mediodorsal Thalamic Nucleus
Messenger RNA
Median Eminence
Solitary Nucleus
Locus Coeruleus
Amygdala
Immune Sera
Spinal Cord
Staining and Labeling

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Marksteiner, J., Kirchmair, R., Mahata, S. K., Mahata, M., Fischer-Colbrie, R., Hogue-Angeletti, R., ... Winkler, H. (1993). Distribution of secretoneurin, a peptide derived from secretogranin II, in rat brain: An immunocytochemical and radioimmunological study. Neuroscience, 54(4), 923-944. https://doi.org/10.1016/0306-4522(93)90585-4

Distribution of secretoneurin, a peptide derived from secretogranin II, in rat brain : An immunocytochemical and radioimmunological study. / Marksteiner, J.; Kirchmair, R.; Mahata, S. K.; Mahata, M.; Fischer-Colbrie, R.; Hogue-Angeletti, R.; Saria, A.; Winkler, H.

In: Neuroscience, Vol. 54, No. 4, 1993, p. 923-944.

Research output: Contribution to journalArticle

Marksteiner, J, Kirchmair, R, Mahata, SK, Mahata, M, Fischer-Colbrie, R, Hogue-Angeletti, R, Saria, A & Winkler, H 1993, 'Distribution of secretoneurin, a peptide derived from secretogranin II, in rat brain: An immunocytochemical and radioimmunological study', Neuroscience, vol. 54, no. 4, pp. 923-944. https://doi.org/10.1016/0306-4522(93)90585-4
Marksteiner, J. ; Kirchmair, R. ; Mahata, S. K. ; Mahata, M. ; Fischer-Colbrie, R. ; Hogue-Angeletti, R. ; Saria, A. ; Winkler, H. / Distribution of secretoneurin, a peptide derived from secretogranin II, in rat brain : An immunocytochemical and radioimmunological study. In: Neuroscience. 1993 ; Vol. 54, No. 4. pp. 923-944.
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