Distinctive flow histogram pattern in molar pregnancies with elevated maternal serum human chorionic gonadotropin levels

T. J. Bocklage, Harriet O. Smith, S. A. Bartow

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Background. Flow cytometric analysis of trophoblastic tissue has shown that most partial hydatidiform moles (PMs) are triploid, whereas most complete moles (CMs) are diploid or tetraploid. Ploidy analysis can support a diagnosis of CM or PM. However, in some cases, a precise diagnosis cannot be rendered. Methods. This study examined DNA flow histograms in 86 cases of histologically diagnosed moles and nonmoles to identify patterns specific to moles to eliminate indeterminate diagnoses. Forty hydropic abortions, 17 CMs, and 29 PMs were analyzed, and results were correlated with microscopic appearance and maternal serum human chorionic gonadotropin (HCG) levels. Results. Analysis of nondiploid histologic moles in which the initial maternal serum HCG level was greater than 150,000 mIU/ml showed similar histograms in 12 of 14 cases. In these 12 specimens, a distinct aneuploid peak could not be delineated from multiple cell populations between the G0/G1 and G2/M or G0/G1(diploid) and G0/G1(aneuploid) peaks. This commonly appeared as a slope rising toward the tetraploid region. S-phase fraction values showed a trend toward higher values in the moles versus nonmoles, but the difference was not statistically significant. Conclusions. This sloping histogram pattern may reflect progression from a single aneuploid to multiple aneuploid populations. Its statistically significant correlation (P < 0.001) with high maternal serum HCG values suggests the presence of a highly metabolically active population of aneuploid trophoblast. Because it appears specific to nondiploid moles, recognition of the pattern will aid in the distinction of mole from hydropic spontaneous abortion.

Original languageEnglish (US)
Pages (from-to)2782-2790
Number of pages9
JournalCancer
Volume73
Issue number11
DOIs
StatePublished - 1994
Externally publishedYes

Fingerprint

Hydatidiform Mole
Aneuploidy
Chorionic Gonadotropin
Mothers
Serum
Tetraploidy
Diploidy
Edema
Population
Triploidy
Ploidies
Trophoblasts
Spontaneous Abortion
S Phase
DNA

Keywords

  • abortion
  • flow cytometry
  • gonadotropins, chorionic
  • hydatidiform mole
  • ploidies

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Distinctive flow histogram pattern in molar pregnancies with elevated maternal serum human chorionic gonadotropin levels. / Bocklage, T. J.; Smith, Harriet O.; Bartow, S. A.

In: Cancer, Vol. 73, No. 11, 1994, p. 2782-2790.

Research output: Contribution to journalArticle

@article{6ccb0c8ac52d4bceb91beee4d5aefa9b,
title = "Distinctive flow histogram pattern in molar pregnancies with elevated maternal serum human chorionic gonadotropin levels",
abstract = "Background. Flow cytometric analysis of trophoblastic tissue has shown that most partial hydatidiform moles (PMs) are triploid, whereas most complete moles (CMs) are diploid or tetraploid. Ploidy analysis can support a diagnosis of CM or PM. However, in some cases, a precise diagnosis cannot be rendered. Methods. This study examined DNA flow histograms in 86 cases of histologically diagnosed moles and nonmoles to identify patterns specific to moles to eliminate indeterminate diagnoses. Forty hydropic abortions, 17 CMs, and 29 PMs were analyzed, and results were correlated with microscopic appearance and maternal serum human chorionic gonadotropin (HCG) levels. Results. Analysis of nondiploid histologic moles in which the initial maternal serum HCG level was greater than 150,000 mIU/ml showed similar histograms in 12 of 14 cases. In these 12 specimens, a distinct aneuploid peak could not be delineated from multiple cell populations between the G0/G1 and G2/M or G0/G1(diploid) and G0/G1(aneuploid) peaks. This commonly appeared as a slope rising toward the tetraploid region. S-phase fraction values showed a trend toward higher values in the moles versus nonmoles, but the difference was not statistically significant. Conclusions. This sloping histogram pattern may reflect progression from a single aneuploid to multiple aneuploid populations. Its statistically significant correlation (P < 0.001) with high maternal serum HCG values suggests the presence of a highly metabolically active population of aneuploid trophoblast. Because it appears specific to nondiploid moles, recognition of the pattern will aid in the distinction of mole from hydropic spontaneous abortion.",
keywords = "abortion, flow cytometry, gonadotropins, chorionic, hydatidiform mole, ploidies",
author = "Bocklage, {T. J.} and Smith, {Harriet O.} and Bartow, {S. A.}",
year = "1994",
doi = "10.1002/1097-0142(19940601)73:11<2782::AID-CNCR2820731122>3.0.CO;2-A",
language = "English (US)",
volume = "73",
pages = "2782--2790",
journal = "Cancer",
issn = "0008-543X",
publisher = "John Wiley and Sons Inc.",
number = "11",

}

TY - JOUR

T1 - Distinctive flow histogram pattern in molar pregnancies with elevated maternal serum human chorionic gonadotropin levels

AU - Bocklage, T. J.

AU - Smith, Harriet O.

AU - Bartow, S. A.

PY - 1994

Y1 - 1994

N2 - Background. Flow cytometric analysis of trophoblastic tissue has shown that most partial hydatidiform moles (PMs) are triploid, whereas most complete moles (CMs) are diploid or tetraploid. Ploidy analysis can support a diagnosis of CM or PM. However, in some cases, a precise diagnosis cannot be rendered. Methods. This study examined DNA flow histograms in 86 cases of histologically diagnosed moles and nonmoles to identify patterns specific to moles to eliminate indeterminate diagnoses. Forty hydropic abortions, 17 CMs, and 29 PMs were analyzed, and results were correlated with microscopic appearance and maternal serum human chorionic gonadotropin (HCG) levels. Results. Analysis of nondiploid histologic moles in which the initial maternal serum HCG level was greater than 150,000 mIU/ml showed similar histograms in 12 of 14 cases. In these 12 specimens, a distinct aneuploid peak could not be delineated from multiple cell populations between the G0/G1 and G2/M or G0/G1(diploid) and G0/G1(aneuploid) peaks. This commonly appeared as a slope rising toward the tetraploid region. S-phase fraction values showed a trend toward higher values in the moles versus nonmoles, but the difference was not statistically significant. Conclusions. This sloping histogram pattern may reflect progression from a single aneuploid to multiple aneuploid populations. Its statistically significant correlation (P < 0.001) with high maternal serum HCG values suggests the presence of a highly metabolically active population of aneuploid trophoblast. Because it appears specific to nondiploid moles, recognition of the pattern will aid in the distinction of mole from hydropic spontaneous abortion.

AB - Background. Flow cytometric analysis of trophoblastic tissue has shown that most partial hydatidiform moles (PMs) are triploid, whereas most complete moles (CMs) are diploid or tetraploid. Ploidy analysis can support a diagnosis of CM or PM. However, in some cases, a precise diagnosis cannot be rendered. Methods. This study examined DNA flow histograms in 86 cases of histologically diagnosed moles and nonmoles to identify patterns specific to moles to eliminate indeterminate diagnoses. Forty hydropic abortions, 17 CMs, and 29 PMs were analyzed, and results were correlated with microscopic appearance and maternal serum human chorionic gonadotropin (HCG) levels. Results. Analysis of nondiploid histologic moles in which the initial maternal serum HCG level was greater than 150,000 mIU/ml showed similar histograms in 12 of 14 cases. In these 12 specimens, a distinct aneuploid peak could not be delineated from multiple cell populations between the G0/G1 and G2/M or G0/G1(diploid) and G0/G1(aneuploid) peaks. This commonly appeared as a slope rising toward the tetraploid region. S-phase fraction values showed a trend toward higher values in the moles versus nonmoles, but the difference was not statistically significant. Conclusions. This sloping histogram pattern may reflect progression from a single aneuploid to multiple aneuploid populations. Its statistically significant correlation (P < 0.001) with high maternal serum HCG values suggests the presence of a highly metabolically active population of aneuploid trophoblast. Because it appears specific to nondiploid moles, recognition of the pattern will aid in the distinction of mole from hydropic spontaneous abortion.

KW - abortion

KW - flow cytometry

KW - gonadotropins, chorionic

KW - hydatidiform mole

KW - ploidies

UR - http://www.scopus.com/inward/record.url?scp=0028319299&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028319299&partnerID=8YFLogxK

U2 - 10.1002/1097-0142(19940601)73:11<2782::AID-CNCR2820731122>3.0.CO;2-A

DO - 10.1002/1097-0142(19940601)73:11<2782::AID-CNCR2820731122>3.0.CO;2-A

M3 - Article

VL - 73

SP - 2782

EP - 2790

JO - Cancer

JF - Cancer

SN - 0008-543X

IS - 11

ER -