Distinct cytokine profiles of neonatal natural killer T cells after expansion with subsets of dendritic cells

Norimitsu Kadowaki, Svetlana Antonenko, Stephen Ho, Marie Clotilde Rissoan, Vassili Soumelis, Steven A. Porcelli, Lewis L. Lanier, Yong Jun Liu

Research output: Contribution to journalArticle

144 Citations (Scopus)

Abstract

Natural killer T (NKT) cells are a highly conserved subset of T cells that have been shown to play a critical role in suppressing T helper cell type 1 - mediated autoimmune diseases and graft versus host disease in an interleukin (IL)-4 - dependent manner. Thus, it is important to understand how the development of IL-4 - versus interferon (IFN)-γ - producing NKT cells is regulated. Here, we show that NKT cells from adult blood and those from cord blood undergo massive expansion in cell numbers (500-70,000-fold) during a 4-wk culture with IL-2, IL-7, phytohemagglutinin, anti-CD3, and anti-CD28 mAbs. Unlike adult NKT cells that preferentially produce both IL-4 and IFN-γ, neonatal NKT cells preferentially produce IL-4 after polyclonal activation. Addition of type 2 dendritic cells (DC2) enhances the development of neonatal NKT cells into IL-4+IFN-γ- NKT2 cells, whereas addition of type 1 dendritic cells (DC1) induces polarization towards IL-4-IFN-γ+ NKT1 cells. Adult NKT cells display limited plasticity for polarization induced by DC1 or DC2. Thus, newly generated NKT cells may possess the potent ability to develop into IL-4+IFN-γ- NKT2 cells in response to appropriate stimuli and may thereafter acquire the tendency to produce both IL-4 and IFN-γ.

Original languageEnglish (US)
Pages (from-to)1221-1226
Number of pages6
JournalJournal of Experimental Medicine
Volume193
Issue number10
DOIs
StatePublished - May 21 2001

Fingerprint

Natural Killer T-Cells
Interleukin-4
Dendritic Cells
Interferons
Cytokines
Interleukin-7
Th1 Cells
T-Lymphocyte Subsets
Phytohemagglutinins
Graft vs Host Disease
Fetal Blood
Autoimmune Diseases
Interleukin-2
Cell Count

Keywords

  • Autoimmune diseases
  • Cord blood
  • Graft versus host disease
  • Interferon γ
  • Interleukin 4

ASJC Scopus subject areas

  • Immunology

Cite this

Distinct cytokine profiles of neonatal natural killer T cells after expansion with subsets of dendritic cells. / Kadowaki, Norimitsu; Antonenko, Svetlana; Ho, Stephen; Rissoan, Marie Clotilde; Soumelis, Vassili; Porcelli, Steven A.; Lanier, Lewis L.; Liu, Yong Jun.

In: Journal of Experimental Medicine, Vol. 193, No. 10, 21.05.2001, p. 1221-1226.

Research output: Contribution to journalArticle

Kadowaki, Norimitsu ; Antonenko, Svetlana ; Ho, Stephen ; Rissoan, Marie Clotilde ; Soumelis, Vassili ; Porcelli, Steven A. ; Lanier, Lewis L. ; Liu, Yong Jun. / Distinct cytokine profiles of neonatal natural killer T cells after expansion with subsets of dendritic cells. In: Journal of Experimental Medicine. 2001 ; Vol. 193, No. 10. pp. 1221-1226.
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