Direct photoaffinity labeling of tubulin with taxol

Srinivasa Rao, Susan Band Horwitz, Israel Ringel

Research output: Contribution to journalArticle

146 Citations (Scopus)

Abstract

Background: Taxol is a potent inhibitor of the replication of eukaryotic cells and has significant antitumor activity in human malignancies. The drug induces the formation of bundles of stable microtubules and blocks cells in the mitotic phase of the cell cycle. In vitro, taxol enhances the polymerization of tubulin to microtubules that are resistant to depolymerization. Although it is evident that taxol interacts with the tubulin-microtubule system, no information has been available on the binding site for the drug on the microtubule. Purpose: Our purpose was to determine if taxol binds to one or both of the tubulin subunits. Methods: In the absence of a photoaffinity-labeled analogue of taxol, [3H]taxol was used directly to photolabel tubulin. A complex of microtubule protein and [3H]taxol was irradiated by ultraviolet light and analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Results and Conclusions: The radio-labeled drug preferentially binds covalently to the β-subunit of tubulin, and the binding can be competed with unlabeled taxol. Implications: This observation is the first step in a study to determine the binding site for taxol on the microtubule.

Original languageEnglish (US)
Pages (from-to)785-788
Number of pages4
JournalJournal of the National Cancer Institute
Volume84
Issue number10
StatePublished - May 20 1992

Fingerprint

Microtubules
Binding sites
Tubulin
Paclitaxel
Labeling
Depolymerization
Sodium dodecyl sulfate
Polyacrylates
Electrophoresis
Drugs
Information systems
Gels
Polymerization
Cells
Proteins
Cell
Cell Cycle
Binding Sites
Ultraviolet
Sodium

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Radiology Nuclear Medicine and imaging

Cite this

Direct photoaffinity labeling of tubulin with taxol. / Rao, Srinivasa; Band Horwitz, Susan; Ringel, Israel.

In: Journal of the National Cancer Institute, Vol. 84, No. 10, 20.05.1992, p. 785-788.

Research output: Contribution to journalArticle

Rao, Srinivasa ; Band Horwitz, Susan ; Ringel, Israel. / Direct photoaffinity labeling of tubulin with taxol. In: Journal of the National Cancer Institute. 1992 ; Vol. 84, No. 10. pp. 785-788.
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