Diphenyl diselenide reverses gastric lesions in rats: Involvement of oxidative stress

R. P. Ineu, M. E. Pereira, Michael Aschner, C. W. Nogueira, G. Zeni, J. B T Rocha

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

The aim of the present study was to evaluate if diphenyl diselenide administered by oral route was effective in restoring gastric lesions induced by ethanol. The possible involvement of oxidative stress in diphenyl diselenide antiulcer effect was also evaluated. Different doses of diphenyl diselenide (dissolved in soya bean oil, 1 mL/kg) were administered orally 1 h before (pre-treatment study) or 1 h after ethanol (70%, v/v, 2 mL/kg, post-treatment study). Ulcer lesions were quantified 1 h after ethanol administration (pre-treatment protocol) or 1 h after diphenyl diselenide study (post-treatment protocol). Diphenyl diselenide (0.1-10 mg/kg or 0.32-32 μmol/kg), when administered previously or posteriorly prevented and reversed respectively, the development of gastric lesions induced by ethanol in rats. A number of markers of oxidative stress were examined in rat stomach including thiobarbituric acid reactive species (TBARS), catalase (CAT), superoxide dismutase (SOD), non-protein thiol groups (NPSH) and ascorbic acid. In addition to attenuating the gastric lesions, low doses of diphenyl diselenide prevented (pre-treatment) or reversed (post-treatment) the ethanol-induced changes in TBARS, SOD activity and ascorbic acid content. In conclusion, the present data reveal that diphenyl diselenide, administered by oral route, possesses an antiulcer effect by modulating antioxidant mechanisms.

Original languageEnglish (US)
Pages (from-to)3023-3029
Number of pages7
JournalFood and Chemical Toxicology
Volume46
Issue number9
DOIs
StatePublished - 2008
Externally publishedYes

Fingerprint

Oxidative stress
lesions (animal)
biphenyl
Rats
Stomach
stomach
Oxidative Stress
oxidative stress
Ethanol
rats
ethanol
anti-ulcer activity
pretreatment
thiobarbituric acid
Clinical Protocols
Ascorbic Acid
Superoxide Dismutase
mouth
superoxide dismutase
ascorbic acid

Keywords

  • Antioxidant
  • Ethanol
  • Gastric lesion
  • Oxidative stress
  • Selenium

ASJC Scopus subject areas

  • Food Science
  • Toxicology

Cite this

Diphenyl diselenide reverses gastric lesions in rats : Involvement of oxidative stress. / Ineu, R. P.; Pereira, M. E.; Aschner, Michael; Nogueira, C. W.; Zeni, G.; Rocha, J. B T.

In: Food and Chemical Toxicology, Vol. 46, No. 9, 2008, p. 3023-3029.

Research output: Contribution to journalArticle

Ineu, R. P. ; Pereira, M. E. ; Aschner, Michael ; Nogueira, C. W. ; Zeni, G. ; Rocha, J. B T. / Diphenyl diselenide reverses gastric lesions in rats : Involvement of oxidative stress. In: Food and Chemical Toxicology. 2008 ; Vol. 46, No. 9. pp. 3023-3029.
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