Differentiation of pancreatic epithelial progenitor cells into hepatocytes following transplantation into rat liver

Mariana D. Dabeva, Seong Gyu Hwang, Srinivasa Rao G Vasa, Ethel Hurston, Phyllis M. Novikoff, Douglas C. Hixson, Sanjeev Gupta, David A. Shafritz

Research output: Contribution to journalArticle

172 Citations (Scopus)

Abstract

The ability to identify, isolate, and transplant progenitor cells from solid tissues would greatly facilitate the treatment of diseases currently requiring whole organ transplantation. In this study, cell fractions enriched in candidate epithelial progenitor cells from the rat pancreas were isolated and transplanted into the liver of an inbred strain of Fischer rats. Using a dipeptidyl dipeptidase IV genetic marker system to follow the fate of transplanted cells in conjunction with albumin gene expression, we provide conclusive evidence that, after transplantation to the liver, epithelial progenitor cells from the pancreas differentiate into hepatocytes, express liver-specific proteins, and become fully integrated into the liver parenchymal structure. These studies demonstrate the presence of multipotent progenitor cells in the adult pancreas and establish a role for the liver micro-environment in the terminal differentiation of epithelial cells of foregut origin. They further suggest that such progenitor cells might be useful in studies of organ repopulation following acute or chronic liver injury.

Original languageEnglish (US)
Pages (from-to)7356-7361
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume94
Issue number14
DOIs
StatePublished - Jul 8 1997

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Hepatocytes
Stem Cells
Transplantation
Epithelial Cells
Liver
Pancreas
Inbred Strains Rats
Inbred F344 Rats
Organ Transplantation
Genetic Markers
Liver Transplantation
Albumins
Transplants
Gene Expression
Wounds and Injuries
Proteins
Therapeutics

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Differentiation of pancreatic epithelial progenitor cells into hepatocytes following transplantation into rat liver. / Dabeva, Mariana D.; Hwang, Seong Gyu; Vasa, Srinivasa Rao G; Hurston, Ethel; Novikoff, Phyllis M.; Hixson, Douglas C.; Gupta, Sanjeev; Shafritz, David A.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, No. 14, 08.07.1997, p. 7356-7361.

Research output: Contribution to journalArticle

Dabeva, Mariana D. ; Hwang, Seong Gyu ; Vasa, Srinivasa Rao G ; Hurston, Ethel ; Novikoff, Phyllis M. ; Hixson, Douglas C. ; Gupta, Sanjeev ; Shafritz, David A. / Differentiation of pancreatic epithelial progenitor cells into hepatocytes following transplantation into rat liver. In: Proceedings of the National Academy of Sciences of the United States of America. 1997 ; Vol. 94, No. 14. pp. 7356-7361.
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