Differentiation of normal and radioresistant prostate cancer xenografts using magnetization transfer-prepared MRI

Wilfred W. Lam, Wendy Oakden, Leedan Murray, Jonathan Klein, Caterina Iorio, Robert A. Screaton, Margaret M. Koletar, William Chu, Stanley K. Liu, Greg J. Stanisz

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5 Scopus citations

Abstract

The ability of MRI to differentiate between normal and radioresistant cancer was investigated in prostate tumour xenografts in mice. Specifically, the process of magnetization exchange between water and other molecules was studied. It was found that magnetization transfer from semisolid macromolecules (MT) and chemical exchange saturation transfer (CEST) combined were significantly different between groups (p < 0.01). Further, the T 2 relaxation of the semisolid macromolecular pool (T 2,B), a parameter specific to MT, was found to be significantly different (p < 0.01). Also significantly different were the rNOE contributions associated with methine groups at -0.9 ppm with a saturation B 1 of 0.5 μT (p < 0.01) and with other aliphatic groups at -3.3 ppm with 0.5 and 2 μT (both p < 0.05). Independently, using a live-cell metabolic assay, normal cells were found to have a greater metabolic rate than radioresistant ones. Thus, MRI provides a novel, in vivo method to quantify the metabolic rate of tumours and predict their radiosensitivity.

Original languageEnglish (US)
Article number10447
JournalScientific reports
Volume8
Issue number1
DOIs
StatePublished - Dec 1 2018
Externally publishedYes

ASJC Scopus subject areas

  • General

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    Lam, W. W., Oakden, W., Murray, L., Klein, J., Iorio, C., Screaton, R. A., Koletar, M. M., Chu, W., Liu, S. K., & Stanisz, G. J. (2018). Differentiation of normal and radioresistant prostate cancer xenografts using magnetization transfer-prepared MRI. Scientific reports, 8(1), [10447]. https://doi.org/10.1038/s41598-018-28731-0