TY - JOUR
T1 - Differential sensitivity of skeletal and fusimotor neurons to Bcl-2-mediated apoptosis during neuromuscular development
AU - Hui, K.
AU - Kucera, J.
AU - Henderson, J. T.
N1 - Funding Information:
Acknowledgements. We thank Dr. Dennis Loh for the gift of bcl-2 null animals generated by his laboratory. We gratefully acknowledge the assistance of Maria Mendez for technical help and support on the morphometric analysis, Jackie Pittman, Douglas Holmyard, and Nancy Good for technical assistance on nerve thin sections. This work was supported by Rick Hansen Institute, ALS Society of Canada, and Canadian Institute of Health Research Institute. KH was supported by CIHR/Rx&D Health Research Foundation.
PY - 2008/4
Y1 - 2008/4
N2 - Proper development of the nervous system requires that a carefully controlled balance be maintained between both proliferation and neuronal survival. The process of programmed cell death is believed to play a key role in regulating levels of neuronal survival, in large part through the action of antiapoptotic proteins, such as Bcl-2. Consistent with this, Bcl-2 has been shown to be a key regulator of apoptotic signaling in post-mitotic neurons. However, we still know remarkably little regarding the role that Bcl-2 plays in regulating the survival of specific motor neuron populations. In the present study, we have examined somatic motor neurons of the lumbar spinal cord, and branchiomotor neurons of the facial nucleus in bcl-2-null mice to determine the differential dependence among motor neuron populations with respect to Bcl-2-mediated survival. Examination of neuronal and axon number, axonal area, and the distribution of axonal loss in bcl-2-null mice demonstrates that, in contrast to the great majority of alpha motor neurons, gamma motor neurons exhibit a unique dependence upon bcl-2 for survival. These results demonstrate, for the first time, the connection between Bcl-2 expression, motor neuron survival, and the establishment of different motor populations.
AB - Proper development of the nervous system requires that a carefully controlled balance be maintained between both proliferation and neuronal survival. The process of programmed cell death is believed to play a key role in regulating levels of neuronal survival, in large part through the action of antiapoptotic proteins, such as Bcl-2. Consistent with this, Bcl-2 has been shown to be a key regulator of apoptotic signaling in post-mitotic neurons. However, we still know remarkably little regarding the role that Bcl-2 plays in regulating the survival of specific motor neuron populations. In the present study, we have examined somatic motor neurons of the lumbar spinal cord, and branchiomotor neurons of the facial nucleus in bcl-2-null mice to determine the differential dependence among motor neuron populations with respect to Bcl-2-mediated survival. Examination of neuronal and axon number, axonal area, and the distribution of axonal loss in bcl-2-null mice demonstrates that, in contrast to the great majority of alpha motor neurons, gamma motor neurons exhibit a unique dependence upon bcl-2 for survival. These results demonstrate, for the first time, the connection between Bcl-2 expression, motor neuron survival, and the establishment of different motor populations.
UR - http://www.scopus.com/inward/record.url?scp=40949136435&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=40949136435&partnerID=8YFLogxK
U2 - 10.1038/sj.cdd.4402294
DO - 10.1038/sj.cdd.4402294
M3 - Article
C2 - 18097449
AN - SCOPUS:40949136435
SN - 1350-9047
VL - 15
SP - 691
EP - 699
JO - Cell Death and Differentiation
JF - Cell Death and Differentiation
IS - 4
ER -