Differential expression between synaptic vesicle proteins and presynaptic plasma membrane proteins in the anterior horn of amyotrophic lateral sclerosis

Akito Ikemoto, Shinichi Nakamura, Ichiro Akiguchi, Asao Hirano

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

This study concerns the immunohistochemical investigation of synaptic proteins in the anterior horn of amyotrophic lateral sclerosis (ALS). Antibodies against synapsin 1 and synaptophysin (i.e. synaptic vesicle proteins), and those against syntaxin and the synaptosomal-associated, 25 kDa protein, SNAP25 (i.e. presynaptic plasma membrane proteins) were used for immunostaining, respectively. Lumbar spinal cords from five ALS and eight control patients were examined. In the controls, all four synaptic proteins exhibited fine granular immunoreactivities, distributed throughout the spinal gray matter almost uniformly. In contrast, in all five ALS patients, two of the synaptic vesicle proteins examined decreased in the anterior horn neuropil diffusely, while in the same lumbar segments of these cases the immunoreactivities of the two presynaptic plasma membrane proteins showed no apparent decrease, or were only mildly diminished in the same gray matter area. These results indicate that, during the presynaptic terminal degeneration in the anterior horn of ALS, synaptic vesicle involvement may precede that of the presynaptic plasma membrane.

Original languageEnglish (US)
Pages (from-to)179-187
Number of pages9
JournalActa Neuropathologica
Volume103
Issue number2
DOIs
StatePublished - 2002

Fingerprint

Synaptic Vesicles
Amyotrophic Lateral Sclerosis
Horns
Blood Proteins
Membrane Proteins
Cell Membrane
Spinal Cord
Proteins
Synaptosomal-Associated Protein 25
Synapsins
Qa-SNARE Proteins
Synaptophysin
Neuropil
Presynaptic Terminals
Antibodies
Gray Matter

Keywords

  • Amyotrophic lateral sclerosis
  • Presynaptic terminal
  • Spinal cord
  • Synaptic protein

ASJC Scopus subject areas

  • Clinical Neurology
  • Pathology and Forensic Medicine
  • Neuroscience(all)

Cite this

Differential expression between synaptic vesicle proteins and presynaptic plasma membrane proteins in the anterior horn of amyotrophic lateral sclerosis. / Ikemoto, Akito; Nakamura, Shinichi; Akiguchi, Ichiro; Hirano, Asao.

In: Acta Neuropathologica, Vol. 103, No. 2, 2002, p. 179-187.

Research output: Contribution to journalArticle

Ikemoto, Akito ; Nakamura, Shinichi ; Akiguchi, Ichiro ; Hirano, Asao. / Differential expression between synaptic vesicle proteins and presynaptic plasma membrane proteins in the anterior horn of amyotrophic lateral sclerosis. In: Acta Neuropathologica. 2002 ; Vol. 103, No. 2. pp. 179-187.
@article{2325c94e47914fafbfc06c282f5bed04,
title = "Differential expression between synaptic vesicle proteins and presynaptic plasma membrane proteins in the anterior horn of amyotrophic lateral sclerosis",
abstract = "This study concerns the immunohistochemical investigation of synaptic proteins in the anterior horn of amyotrophic lateral sclerosis (ALS). Antibodies against synapsin 1 and synaptophysin (i.e. synaptic vesicle proteins), and those against syntaxin and the synaptosomal-associated, 25 kDa protein, SNAP25 (i.e. presynaptic plasma membrane proteins) were used for immunostaining, respectively. Lumbar spinal cords from five ALS and eight control patients were examined. In the controls, all four synaptic proteins exhibited fine granular immunoreactivities, distributed throughout the spinal gray matter almost uniformly. In contrast, in all five ALS patients, two of the synaptic vesicle proteins examined decreased in the anterior horn neuropil diffusely, while in the same lumbar segments of these cases the immunoreactivities of the two presynaptic plasma membrane proteins showed no apparent decrease, or were only mildly diminished in the same gray matter area. These results indicate that, during the presynaptic terminal degeneration in the anterior horn of ALS, synaptic vesicle involvement may precede that of the presynaptic plasma membrane.",
keywords = "Amyotrophic lateral sclerosis, Presynaptic terminal, Spinal cord, Synaptic protein",
author = "Akito Ikemoto and Shinichi Nakamura and Ichiro Akiguchi and Asao Hirano",
year = "2002",
doi = "10.1007/s004010100449",
language = "English (US)",
volume = "103",
pages = "179--187",
journal = "Acta Neuropathologica",
issn = "0001-6322",
publisher = "Springer Verlag",
number = "2",

}

TY - JOUR

T1 - Differential expression between synaptic vesicle proteins and presynaptic plasma membrane proteins in the anterior horn of amyotrophic lateral sclerosis

AU - Ikemoto, Akito

AU - Nakamura, Shinichi

AU - Akiguchi, Ichiro

AU - Hirano, Asao

PY - 2002

Y1 - 2002

N2 - This study concerns the immunohistochemical investigation of synaptic proteins in the anterior horn of amyotrophic lateral sclerosis (ALS). Antibodies against synapsin 1 and synaptophysin (i.e. synaptic vesicle proteins), and those against syntaxin and the synaptosomal-associated, 25 kDa protein, SNAP25 (i.e. presynaptic plasma membrane proteins) were used for immunostaining, respectively. Lumbar spinal cords from five ALS and eight control patients were examined. In the controls, all four synaptic proteins exhibited fine granular immunoreactivities, distributed throughout the spinal gray matter almost uniformly. In contrast, in all five ALS patients, two of the synaptic vesicle proteins examined decreased in the anterior horn neuropil diffusely, while in the same lumbar segments of these cases the immunoreactivities of the two presynaptic plasma membrane proteins showed no apparent decrease, or were only mildly diminished in the same gray matter area. These results indicate that, during the presynaptic terminal degeneration in the anterior horn of ALS, synaptic vesicle involvement may precede that of the presynaptic plasma membrane.

AB - This study concerns the immunohistochemical investigation of synaptic proteins in the anterior horn of amyotrophic lateral sclerosis (ALS). Antibodies against synapsin 1 and synaptophysin (i.e. synaptic vesicle proteins), and those against syntaxin and the synaptosomal-associated, 25 kDa protein, SNAP25 (i.e. presynaptic plasma membrane proteins) were used for immunostaining, respectively. Lumbar spinal cords from five ALS and eight control patients were examined. In the controls, all four synaptic proteins exhibited fine granular immunoreactivities, distributed throughout the spinal gray matter almost uniformly. In contrast, in all five ALS patients, two of the synaptic vesicle proteins examined decreased in the anterior horn neuropil diffusely, while in the same lumbar segments of these cases the immunoreactivities of the two presynaptic plasma membrane proteins showed no apparent decrease, or were only mildly diminished in the same gray matter area. These results indicate that, during the presynaptic terminal degeneration in the anterior horn of ALS, synaptic vesicle involvement may precede that of the presynaptic plasma membrane.

KW - Amyotrophic lateral sclerosis

KW - Presynaptic terminal

KW - Spinal cord

KW - Synaptic protein

UR - http://www.scopus.com/inward/record.url?scp=0036934956&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036934956&partnerID=8YFLogxK

U2 - 10.1007/s004010100449

DO - 10.1007/s004010100449

M3 - Article

C2 - 11810185

AN - SCOPUS:0036934956

VL - 103

SP - 179

EP - 187

JO - Acta Neuropathologica

JF - Acta Neuropathologica

SN - 0001-6322

IS - 2

ER -