Differential epigenome-wide DNA methylation patterns in childhood obesity-associated asthma

Research output: Contribution to journalArticle

52 Scopus citations

Abstract

While DNA methylation plays a role in T-helper (Th) cell maturation, its potential dysregulation in the non-atopic Th1-polarized systemic inflammation observed in obesity-associated asthma is unknown. We studied DNA methylation epigenome-wide in peripheral blood mononuclear cells (PBMCs) from 8 obese asthmatic pre-adolescent children and compared it to methylation in PBMCs from 8 children with asthma alone, obesity alone and healthy controls. Differentially methylated loci implicated certain biologically relevant molecules and pathways. PBMCs from obese asthmatic children had distinctive DNA methylation patterns, with decreased promoter methylation of CCL5, IL2RA and TBX21, genes encoding proteins linked with Th1 polarization, and increased promoter methylation of FCER2, a low-affinity receptor for IgE, and of TGFB1, inhibitor of Th cell activation. T-cell signaling and macrophage activation were the two primary pathways that were selectively hypomethylated in obese asthmatics. These findings suggest that dysregulated DNA methylation is associated with non-atopic inflammation observed in pediatric obesity-associated asthma.

Original languageEnglish (US)
Article number2164
JournalScientific reports
Volume3
DOIs
StatePublished - Jul 24 2013

ASJC Scopus subject areas

  • General

Fingerprint Dive into the research topics of 'Differential epigenome-wide DNA methylation patterns in childhood obesity-associated asthma'. Together they form a unique fingerprint.

  • Cite this