Dietary salt induces transcription of the prostaglandin transporter gene in renal collecting ducts

Yuling Chi, Michael L. Pucci, Victor L. Schuster

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Prostaglandin E2 (PGE2) plays an important role in maintaining body fluid homeostasis by activating its receptors on the renal collecting duct (CD) to stimulate renal Na+ and water excretion. The PG carrier prostaglandin transporter (PGT) is expressed on the CD apical membrane, where it mediates PG reuptake as part of the termination of autocrine PG signaling. Here we tested the hypothesis that dietary salt loading regulates PGT gene transcription in renal CDs. We placed green fluorescence protein (GFP) under control of 3.3 kb of the mouse PGT promoter and injected this construct into the pronuclei of fertilized FVB mouse eggs. Four of thirty-eight offspring were GFP positive by genotyping. We extensively characterized one (no. 29) PGT-GFP transgenic mouse line. On microscopic examination, GFP was expressed in CDs as determined by their expression of aquaporin-2. We fed mice a low (0.03% NaCl)-, normal (0.3% NaCl)-, or high-salt (3% NaCl) diet for 2 wk and quantified CD GFP expression. The average number of GFP-positive CD cells per microscopic section varied directly with dietary salt intake. Compared with mice on the control (0.3% sodium) diet, mice on a low-sodium (0.03%) diet had reduced numbers of GFP-positive cells (71% of control, P < 0.001), whereas mice on a high-sodium (3%) diet had increased numbers of GFPpositive cells (139% of control, P < 0.001). This increase in apparent CD PGT transcription resulted in a 51-55% increase (P < 0.001) in whole kidney PGT mRNA levels as determined by real-time PCR. The regulation of PG signal termination via reuptake represents a new pathway for controlling renal Na+ balance.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Renal Physiology
Volume295
Issue number3
DOIs
StatePublished - Sep 2008

Fingerprint

Prostaglandins
Salts
Fluorescence
Kidney
Genes
Proteins
Diet
Sodium
Autocrine Communication
Aquaporin 2
Sodium-Restricted Diet
Body Fluids
Dinoprostone
Eggs
Transgenic Mice
Real-Time Polymerase Chain Reaction
Homeostasis
Cell Count
Messenger RNA
Membranes

Keywords

  • Dietary sodium
  • Natriuresis
  • Organic anion transport
  • Transgenic mice

ASJC Scopus subject areas

  • Physiology
  • Urology

Cite this

Dietary salt induces transcription of the prostaglandin transporter gene in renal collecting ducts. / Chi, Yuling; Pucci, Michael L.; Schuster, Victor L.

In: American Journal of Physiology - Renal Physiology, Vol. 295, No. 3, 09.2008.

Research output: Contribution to journalArticle

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abstract = "Prostaglandin E2 (PGE2) plays an important role in maintaining body fluid homeostasis by activating its receptors on the renal collecting duct (CD) to stimulate renal Na+ and water excretion. The PG carrier prostaglandin transporter (PGT) is expressed on the CD apical membrane, where it mediates PG reuptake as part of the termination of autocrine PG signaling. Here we tested the hypothesis that dietary salt loading regulates PGT gene transcription in renal CDs. We placed green fluorescence protein (GFP) under control of 3.3 kb of the mouse PGT promoter and injected this construct into the pronuclei of fertilized FVB mouse eggs. Four of thirty-eight offspring were GFP positive by genotyping. We extensively characterized one (no. 29) PGT-GFP transgenic mouse line. On microscopic examination, GFP was expressed in CDs as determined by their expression of aquaporin-2. We fed mice a low (0.03{\%} NaCl)-, normal (0.3{\%} NaCl)-, or high-salt (3{\%} NaCl) diet for 2 wk and quantified CD GFP expression. The average number of GFP-positive CD cells per microscopic section varied directly with dietary salt intake. Compared with mice on the control (0.3{\%} sodium) diet, mice on a low-sodium (0.03{\%}) diet had reduced numbers of GFP-positive cells (71{\%} of control, P < 0.001), whereas mice on a high-sodium (3{\%}) diet had increased numbers of GFPpositive cells (139{\%} of control, P < 0.001). This increase in apparent CD PGT transcription resulted in a 51-55{\%} increase (P < 0.001) in whole kidney PGT mRNA levels as determined by real-time PCR. The regulation of PG signal termination via reuptake represents a new pathway for controlling renal Na+ balance.",
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