Abstract
A high saturated fat diet induces free cholesterol and phospholipid accumulation in the plasma of phospholipid transfer protein (Pltp)-deficient mice. In this study, we examined the atherogenic consequence of this phenomenon and investigated the possible mechanism(s). Pltp KO/Apoe KO mice that were fed a coconut oil-enriched high-fat diet (COD) for 7 weeks had higher plasma free cholesterol (149%), phospholipids (15%), and sphingomyelin (54%) than Apoe KO controls. In contrast to chow-fed animals, COD-fed Pltp KO/Apoe KO mice had the same atherosclerotic lesion size as that of Apoe KO mice. Similar to Pltp KO mice, plasma from COD-fed Pltp KO /Apoe KO mice contained VLDL/LDL-sized lamellar particles. Bile measurement indicated that COD-fed Pltp KO mice have 33% less hepatic cholesterol output than controls. In conclusion, COD-fed, Pltp-deficient mice are no longer protected from atherosclerosis and have impaired biliary lipid secretion, which is associated with free cholesterol and phospholipid accumulation.
Original language | English (US) |
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Pages (from-to) | 2993-3002 |
Number of pages | 10 |
Journal | Journal of Lipid Research |
Volume | 51 |
Issue number | 10 |
DOIs | |
State | Published - Oct 2010 |
Externally published | Yes |
Keywords
- Atherosclerosis
- Bile production
- Free cholesterol
- Saturated fat-based diet
ASJC Scopus subject areas
- Biochemistry
- Endocrinology
- Cell Biology