Diet-induced lipid accumulation in phospholipid transfer protein-deficient mice: Its atherogenicity and potential mechanism

Calvin Yeang, Shucun Qin, Kailian Chen, David Q.H. Wang, Xian Cheng Jiang

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

A high saturated fat diet induces free cholesterol and phospholipid accumulation in the plasma of phospholipid transfer protein (Pltp)-deficient mice. In this study, we examined the atherogenic consequence of this phenomenon and investigated the possible mechanism(s). Pltp KO/Apoe KO mice that were fed a coconut oil-enriched high-fat diet (COD) for 7 weeks had higher plasma free cholesterol (149%), phospholipids (15%), and sphingomyelin (54%) than Apoe KO controls. In contrast to chow-fed animals, COD-fed Pltp KO/Apoe KO mice had the same atherosclerotic lesion size as that of Apoe KO mice. Similar to Pltp KO mice, plasma from COD-fed Pltp KO /Apoe KO mice contained VLDL/LDL-sized lamellar particles. Bile measurement indicated that COD-fed Pltp KO mice have 33% less hepatic cholesterol output than controls. In conclusion, COD-fed, Pltp-deficient mice are no longer protected from atherosclerosis and have impaired biliary lipid secretion, which is associated with free cholesterol and phospholipid accumulation.

Original languageEnglish (US)
Pages (from-to)2993-3002
Number of pages10
JournalJournal of Lipid Research
Volume51
Issue number10
DOIs
StatePublished - Oct 1 2010

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Keywords

  • Atherosclerosis
  • Bile production
  • Free cholesterol
  • Saturated fat-based diet

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology
  • Cell Biology

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