Diego and Prickle regulate frizzled planar cell polarity signalling by competing for Dishevelled binding

Andreas Jenny, Jessica Reynolds-Kenneally, Gishnu Das, Micheal Burnett, Marek Mlodzik

Research output: Contribution to journalArticle

127 Citations (Scopus)

Abstract

Epithelial planar cell polarity (PCP) is evident in the cellular organization of many tissues in vertebrates and invertebrates. In mammals, PCP signalling governs convergent extension during gastrulation and the organization of a wide variety of structures, including the orientation of body hair and sensory hair cells of the inner ear. In Drosophila melanogaster, PCP is manifest in adult tissues, including ommatidial arrangement in the compound eye and hair orientation in wing cells. PCP establishment requires the conserved Frizzled/Dishevelled PCP pathway. Mutations in PCP-pathway-associated genes cause aberrant orientation of body hair or inner-ear sensory cells in mice, or misorientation of ommatidia and wing hair in D. melanogaster. Here we provide mechanistic insight into Frizzled/Dishevelled signalling regulation. We show that the ankyrin-repeat protein Diego binds directly to Dishevelled and promotes Frizzled signalling. Dishevelled can also be bound by the Frizzled PCP antagonist Prickle. Strikingly, Diego and Prickle compete with one another for Dishevelled binding, thereby modulating Frizzled/Dishevelled activity and ensuring tight control over Frizzled PCP signalling.

Original languageEnglish (US)
Pages (from-to)691-697
Number of pages7
JournalNature Cell Biology
Volume7
Issue number7
DOIs
StatePublished - Jul 2005
Externally publishedYes

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Cell Polarity
Hair
Inner Ear
Drosophila melanogaster
Ankyrin Repeat
Gastrulation
Invertebrates
Vertebrates
Mammals
Epithelial Cells
Mutation

ASJC Scopus subject areas

  • Cell Biology

Cite this

Diego and Prickle regulate frizzled planar cell polarity signalling by competing for Dishevelled binding. / Jenny, Andreas; Reynolds-Kenneally, Jessica; Das, Gishnu; Burnett, Micheal; Mlodzik, Marek.

In: Nature Cell Biology, Vol. 7, No. 7, 07.2005, p. 691-697.

Research output: Contribution to journalArticle

Jenny, Andreas ; Reynolds-Kenneally, Jessica ; Das, Gishnu ; Burnett, Micheal ; Mlodzik, Marek. / Diego and Prickle regulate frizzled planar cell polarity signalling by competing for Dishevelled binding. In: Nature Cell Biology. 2005 ; Vol. 7, No. 7. pp. 691-697.
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