Development path and current status of the NANIVID

A new device for cancer cell studies

Waseem Khan Raja, Michael R. Padgen, James K. Williams, Jeffrey Wyckoff, John S. Condeelis, James Castracane

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Citation (Scopus)

Abstract

Cancer cells create a unique microenvironment in vivo which enables migration to distant organs. To better understand the tumor microenvironment, special tools and devices are required to monitor the interactions between different cell types and the effects of particular chemical gradients. This study presents the design and optimization of a new, versatile chemotaxis device called the NANIVID (NANo IntraVital Device). The device is fabricated using BioMEMS techniques and consists of etched and bonded Pyrex substrates, a soluble factor reservoir, fluorescent tracking beads and a microelectrode array for cell quantification. The reservoir contains a customized hydrogel blend loaded with EGF which diffuses out of the hydrogel to create a chemotactic gradient. This reservoir sustains a steady release of growth factor into the surrounding environment for many hours and establishes a concentration gradient that attracts specific cells to the device. In addition to a cell collection tool, the NANIVID can be modified to act as a delivery vehicle for the local generation of alternate soluble factor gradients to initiate controlled changes to the microenvironment such as hypoxia, ECM stiffness and etc. The focus of this study is to design and optimize the new device for wide ranging studies of breast cancer cell dynamics in vitro and ultimately, implantation for in vivo work.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
Volume7929
DOIs
StatePublished - 2011
EventMicrofluidics, BioMEMS, and Medical Microsystems IX - San Francisco, CA, United States
Duration: Jan 23 2011Jan 25 2011

Other

OtherMicrofluidics, BioMEMS, and Medical Microsystems IX
CountryUnited States
CitySan Francisco, CA
Period1/23/111/25/11

Fingerprint

Hydrogel
Hydrogels
Cancer
BioMEMS
cancer
Cells
Military electronic countermeasures
Path
Microelectrodes
Cell
Epidermal Growth Factor
Tumors
Intercellular Signaling Peptides and Proteins
Gradient
Stiffness
gradients
cells
Substrates
Hypoxia
Implantation

Keywords

  • 3D culture
  • Cancer cell migration
  • CoCl
  • Hypoxia
  • Impedance
  • ITO (indium doped tin oxide)
  • Microfabrication

ASJC Scopus subject areas

  • Applied Mathematics
  • Computer Science Applications
  • Electrical and Electronic Engineering
  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics

Cite this

Raja, W. K., Padgen, M. R., Williams, J. K., Wyckoff, J., Condeelis, J. S., & Castracane, J. (2011). Development path and current status of the NANIVID: A new device for cancer cell studies. In Proceedings of SPIE - The International Society for Optical Engineering (Vol. 7929). [79290A] https://doi.org/10.1117/12.883532

Development path and current status of the NANIVID : A new device for cancer cell studies. / Raja, Waseem Khan; Padgen, Michael R.; Williams, James K.; Wyckoff, Jeffrey; Condeelis, John S.; Castracane, James.

Proceedings of SPIE - The International Society for Optical Engineering. Vol. 7929 2011. 79290A.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Raja, WK, Padgen, MR, Williams, JK, Wyckoff, J, Condeelis, JS & Castracane, J 2011, Development path and current status of the NANIVID: A new device for cancer cell studies. in Proceedings of SPIE - The International Society for Optical Engineering. vol. 7929, 79290A, Microfluidics, BioMEMS, and Medical Microsystems IX, San Francisco, CA, United States, 1/23/11. https://doi.org/10.1117/12.883532
Raja WK, Padgen MR, Williams JK, Wyckoff J, Condeelis JS, Castracane J. Development path and current status of the NANIVID: A new device for cancer cell studies. In Proceedings of SPIE - The International Society for Optical Engineering. Vol. 7929. 2011. 79290A https://doi.org/10.1117/12.883532
Raja, Waseem Khan ; Padgen, Michael R. ; Williams, James K. ; Wyckoff, Jeffrey ; Condeelis, John S. ; Castracane, James. / Development path and current status of the NANIVID : A new device for cancer cell studies. Proceedings of SPIE - The International Society for Optical Engineering. Vol. 7929 2011.
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