Development path and current status of the NANIVID: A new device for cancer cell studies

Waseem Khan Raja, Michael R. Padgen, James K. Williams, Jeffrey Wyckoff, John Condeelis, James Castracane

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

Cancer cells create a unique microenvironment in vivo which enables migration to distant organs. To better understand the tumor microenvironment, special tools and devices are required to monitor the interactions between different cell types and the effects of particular chemical gradients. This study presents the design and optimization of a new, versatile chemotaxis device called the NANIVID (NANo IntraVital Device). The device is fabricated using BioMEMS techniques and consists of etched and bonded Pyrex substrates, a soluble factor reservoir, fluorescent tracking beads and a microelectrode array for cell quantification. The reservoir contains a customized hydrogel blend loaded with EGF which diffuses out of the hydrogel to create a chemotactic gradient. This reservoir sustains a steady release of growth factor into the surrounding environment for many hours and establishes a concentration gradient that attracts specific cells to the device. In addition to a cell collection tool, the NANIVID can be modified to act as a delivery vehicle for the local generation of alternate soluble factor gradients to initiate controlled changes to the microenvironment such as hypoxia, ECM stiffness and etc. The focus of this study is to design and optimize the new device for wide ranging studies of breast cancer cell dynamics in vitro and ultimately, implantation for in vivo work.

Original languageEnglish (US)
Title of host publicationMicrofluidics, BioMEMS, and Medical Microsystems IX
DOIs
StatePublished - Jun 9 2011
EventMicrofluidics, BioMEMS, and Medical Microsystems IX - San Francisco, CA, United States
Duration: Jan 23 2011Jan 25 2011

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume7929
ISSN (Print)0277-786X

Other

OtherMicrofluidics, BioMEMS, and Medical Microsystems IX
CountryUnited States
CitySan Francisco, CA
Period1/23/111/25/11

Keywords

  • 3D culture
  • Cancer cell migration
  • CoCl
  • Hypoxia
  • ITO (indium doped tin oxide)
  • Impedance
  • Microfabrication

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

Fingerprint Dive into the research topics of 'Development path and current status of the NANIVID: A new device for cancer cell studies'. Together they form a unique fingerprint.

  • Cite this

    Raja, W. K., Padgen, M. R., Williams, J. K., Wyckoff, J., Condeelis, J., & Castracane, J. (2011). Development path and current status of the NANIVID: A new device for cancer cell studies. In Microfluidics, BioMEMS, and Medical Microsystems IX [79290A] (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 7929). https://doi.org/10.1117/12.883532