Development of microfluidic-based cell collection devices for in vitro and in vivo use

Logan Butt, David R. Entenberg, L. P Madhubhani Hemachandra, Matthew Strohmayer, Patricia Keely, Julio Aguirre-Ghiso, John S. Condeelis, James Castracane

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The NANIVID - or Nano Intravital Device - is an implantable delivery tool designed to locally affect the tumor microenvironment in vivo. This technology is being redesigned and validated as a cell collection tool for the study of metastatic cancer cells. A methodology has been developed to facilitate this transition, consisting of microfluidic analysis of the device microchannels and a series of cell-related collection experiments building up to in vivo collection. Single-chamber designs were first used to qualitatively demonstrate the feasibility of cell collection ex vivo. This was followed by the development and implementation of devices containing a second, negative-control chamber for quantitative analysis. This work sets the foundation for in vivo cancer cell migration studies utilizing the NANIVID.

Original languageEnglish (US)
Title of host publicationMicrofluidics, BioMEMS, and Medical Microsystems XIV
PublisherSPIE
Volume9705
ISBN (Electronic)9781628419399
DOIs
StatePublished - 2016
Event14th SPIE Photonics West Conference: Microfluidics, BioMEMS, and Medical Microsystems - San Francisco, United States
Duration: Feb 13 2016Feb 15 2016

Other

Other14th SPIE Photonics West Conference: Microfluidics, BioMEMS, and Medical Microsystems
CountryUnited States
CitySan Francisco
Period2/13/162/15/16

Fingerprint

Microfluidics
Cells
Equipment and Supplies
Lab-On-A-Chip Devices
Microchannels
cells
Tumors
chambers
cancer
Microfluidic Analytical Techniques
Tumor Microenvironment
microchannels
Chemical analysis
quantitative analysis
Cell Movement
Neoplasms
delivery
tumors
Experiments
methodology

Keywords

  • cancer
  • implantable device
  • invasion
  • metastasis
  • microchannels
  • Microfluidics

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Butt, L., Entenberg, D. R., Hemachandra, L. P. M., Strohmayer, M., Keely, P., Aguirre-Ghiso, J., ... Castracane, J. (2016). Development of microfluidic-based cell collection devices for in vitro and in vivo use. In Microfluidics, BioMEMS, and Medical Microsystems XIV (Vol. 9705). [97051A] SPIE. https://doi.org/10.1117/12.2213316

Development of microfluidic-based cell collection devices for in vitro and in vivo use. / Butt, Logan; Entenberg, David R.; Hemachandra, L. P Madhubhani; Strohmayer, Matthew; Keely, Patricia; Aguirre-Ghiso, Julio; Condeelis, John S.; Castracane, James.

Microfluidics, BioMEMS, and Medical Microsystems XIV. Vol. 9705 SPIE, 2016. 97051A.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Butt, L, Entenberg, DR, Hemachandra, LPM, Strohmayer, M, Keely, P, Aguirre-Ghiso, J, Condeelis, JS & Castracane, J 2016, Development of microfluidic-based cell collection devices for in vitro and in vivo use. in Microfluidics, BioMEMS, and Medical Microsystems XIV. vol. 9705, 97051A, SPIE, 14th SPIE Photonics West Conference: Microfluidics, BioMEMS, and Medical Microsystems, San Francisco, United States, 2/13/16. https://doi.org/10.1117/12.2213316
Butt L, Entenberg DR, Hemachandra LPM, Strohmayer M, Keely P, Aguirre-Ghiso J et al. Development of microfluidic-based cell collection devices for in vitro and in vivo use. In Microfluidics, BioMEMS, and Medical Microsystems XIV. Vol. 9705. SPIE. 2016. 97051A https://doi.org/10.1117/12.2213316
Butt, Logan ; Entenberg, David R. ; Hemachandra, L. P Madhubhani ; Strohmayer, Matthew ; Keely, Patricia ; Aguirre-Ghiso, Julio ; Condeelis, John S. ; Castracane, James. / Development of microfluidic-based cell collection devices for in vitro and in vivo use. Microfluidics, BioMEMS, and Medical Microsystems XIV. Vol. 9705 SPIE, 2016.
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