Development of microfluidic-based cell collection devices for in vitro and in vivo use

Logan Butt; Dave Entenberg; L. P.Madhubhani Hemachandra; Matthew Strohmayer; Patricia Keely; Julio Aguirre-Ghiso; John S. Condeelis; James Castracane

doi:10.1117/12.2213316

Development of microfluidic-based cell collection devices for in vitro and in vivo use

Logan Butt, Dave Entenberg, L. P.Madhubhani Hemachandra, Matthew Strohmayer, Patricia Keely, Julio Aguirre-Ghiso, John S. Condeelis, James Castracane

Anatomy & Structural Biology

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

The NANIVID - or Nano Intravital Device - is an implantable delivery tool designed to locally affect the tumor microenvironment in vivo. This technology is being redesigned and validated as a cell collection tool for the study of metastatic cancer cells. A methodology has been developed to facilitate this transition, consisting of microfluidic analysis of the device microchannels and a series of cell-related collection experiments building up to in vivo collection. Single-chamber designs were first used to qualitatively demonstrate the feasibility of cell collection ex vivo. This was followed by the development and implementation of devices containing a second, negative-control chamber for quantitative analysis. This work sets the foundation for in vivo cancer cell migration studies utilizing the NANIVID.

Original language	English (US)
Title of host publication	Microfluidics, BioMEMS, and Medical Microsystems XIV
Editors	Holger Becker, Bonnie L. Gray
Publisher	SPIE
ISBN (Electronic)	9781628419399
DOIs	https://doi.org/10.1117/12.2213316
State	Published - 2016
Event	14th SPIE Photonics West Conference: Microfluidics, BioMEMS, and Medical Microsystems - San Francisco, United States Duration: Feb 13 2016 → Feb 15 2016

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	9705
ISSN (Print)	1605-7422

Other

Other	14th SPIE Photonics West Conference: Microfluidics, BioMEMS, and Medical Microsystems
Country	United States
City	San Francisco
Period	2/13/16 → 2/15/16

Keywords

Microfluidics
cancer
implantable device
invasion
metastasis
microchannels

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Biomaterials
Atomic and Molecular Physics, and Optics
Radiology Nuclear Medicine and imaging

Access to Document

10.1117/12.2213316

Fingerprint Dive into the research topics of 'Development of microfluidic-based cell collection devices for in vitro and in vivo use'. Together they form a unique fingerprint.

Cite this

Butt, L., Entenberg, D., Hemachandra, L. P. M., Strohmayer, M., Keely, P., Aguirre-Ghiso, J., Condeelis, J. S., & Castracane, J. (2016). Development of microfluidic-based cell collection devices for in vitro and in vivo use. In H. Becker, & B. L. Gray (Eds.), Microfluidics, BioMEMS, and Medical Microsystems XIV [97051A] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 9705). SPIE. https://doi.org/10.1117/12.2213316

Development of microfluidic-based cell collection devices for in vitro and in vivo use. / Butt, Logan; Entenberg, Dave; Hemachandra, L. P.Madhubhani; Strohmayer, Matthew; Keely, Patricia; Aguirre-Ghiso, Julio; Condeelis, John S.; Castracane, James.

Microfluidics, BioMEMS, and Medical Microsystems XIV. ed. / Holger Becker; Bonnie L. Gray. SPIE, 2016. 97051A (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 9705).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Butt, L, Entenberg, D, Hemachandra, LPM, Strohmayer, M, Keely, P, Aguirre-Ghiso, J, Condeelis, JS & Castracane, J 2016, Development of microfluidic-based cell collection devices for in vitro and in vivo use. in H Becker & BL Gray (eds), Microfluidics, BioMEMS, and Medical Microsystems XIV., 97051A, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 9705, SPIE, 14th SPIE Photonics West Conference: Microfluidics, BioMEMS, and Medical Microsystems, San Francisco, United States, 2/13/16. https://doi.org/10.1117/12.2213316

Butt L, Entenberg D, Hemachandra LPM, Strohmayer M, Keely P, Aguirre-Ghiso J et al. Development of microfluidic-based cell collection devices for in vitro and in vivo use. In Becker H, Gray BL, editors, Microfluidics, BioMEMS, and Medical Microsystems XIV. SPIE. 2016. 97051A. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). https://doi.org/10.1117/12.2213316

Butt, Logan ; Entenberg, Dave ; Hemachandra, L. P.Madhubhani ; Strohmayer, Matthew ; Keely, Patricia ; Aguirre-Ghiso, Julio ; Condeelis, John S. ; Castracane, James. / Development of microfluidic-based cell collection devices for in vitro and in vivo use. Microfluidics, BioMEMS, and Medical Microsystems XIV. editor / Holger Becker ; Bonnie L. Gray. SPIE, 2016. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE).

@inproceedings{1251ad5e549d46c98fc2c21a8f5f61cd,

title = "Development of microfluidic-based cell collection devices for in vitro and in vivo use",

abstract = "The NANIVID - or Nano Intravital Device - is an implantable delivery tool designed to locally affect the tumor microenvironment in vivo. This technology is being redesigned and validated as a cell collection tool for the study of metastatic cancer cells. A methodology has been developed to facilitate this transition, consisting of microfluidic analysis of the device microchannels and a series of cell-related collection experiments building up to in vivo collection. Single-chamber designs were first used to qualitatively demonstrate the feasibility of cell collection ex vivo. This was followed by the development and implementation of devices containing a second, negative-control chamber for quantitative analysis. This work sets the foundation for in vivo cancer cell migration studies utilizing the NANIVID.",

keywords = "Microfluidics, cancer, implantable device, invasion, metastasis, microchannels",

author = "Logan Butt and Dave Entenberg and Hemachandra, {L. P.Madhubhani} and Matthew Strohmayer and Patricia Keely and Julio Aguirre-Ghiso and Condeelis, {John S.} and James Castracane",

note = "Funding Information: This work was supported by NIH-NCI grant #U54-CA126511-01 and NSF grant #DBI0922830.; 14th SPIE Photonics West Conference: Microfluidics, BioMEMS, and Medical Microsystems ; Conference date: 13-02-2016 Through 15-02-2016",

year = "2016",

doi = "10.1117/12.2213316",

language = "English (US)",

series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

publisher = "SPIE",

editor = "Holger Becker and Gray, {Bonnie L.}",

booktitle = "Microfluidics, BioMEMS, and Medical Microsystems XIV",

}

TY - GEN

T1 - Development of microfluidic-based cell collection devices for in vitro and in vivo use

AU - Butt, Logan

AU - Entenberg, Dave

AU - Hemachandra, L. P.Madhubhani

AU - Strohmayer, Matthew

AU - Keely, Patricia

AU - Aguirre-Ghiso, Julio

AU - Condeelis, John S.

AU - Castracane, James

N1 - Funding Information: This work was supported by NIH-NCI grant #U54-CA126511-01 and NSF grant #DBI0922830.

PY - 2016

Y1 - 2016

N2 - The NANIVID - or Nano Intravital Device - is an implantable delivery tool designed to locally affect the tumor microenvironment in vivo. This technology is being redesigned and validated as a cell collection tool for the study of metastatic cancer cells. A methodology has been developed to facilitate this transition, consisting of microfluidic analysis of the device microchannels and a series of cell-related collection experiments building up to in vivo collection. Single-chamber designs were first used to qualitatively demonstrate the feasibility of cell collection ex vivo. This was followed by the development and implementation of devices containing a second, negative-control chamber for quantitative analysis. This work sets the foundation for in vivo cancer cell migration studies utilizing the NANIVID.

AB - The NANIVID - or Nano Intravital Device - is an implantable delivery tool designed to locally affect the tumor microenvironment in vivo. This technology is being redesigned and validated as a cell collection tool for the study of metastatic cancer cells. A methodology has been developed to facilitate this transition, consisting of microfluidic analysis of the device microchannels and a series of cell-related collection experiments building up to in vivo collection. Single-chamber designs were first used to qualitatively demonstrate the feasibility of cell collection ex vivo. This was followed by the development and implementation of devices containing a second, negative-control chamber for quantitative analysis. This work sets the foundation for in vivo cancer cell migration studies utilizing the NANIVID.

KW - Microfluidics

KW - cancer

KW - implantable device

KW - invasion

KW - metastasis

KW - microchannels

UR - http://www.scopus.com/inward/record.url?scp=84973926555&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84973926555&partnerID=8YFLogxK

U2 - 10.1117/12.2213316

DO - 10.1117/12.2213316

M3 - Conference contribution

AN - SCOPUS:84973926555

T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

BT - Microfluidics, BioMEMS, and Medical Microsystems XIV

A2 - Becker, Holger

A2 - Gray, Bonnie L.

PB - SPIE

T2 - 14th SPIE Photonics West Conference: Microfluidics, BioMEMS, and Medical Microsystems

Y2 - 13 February 2016 through 15 February 2016

ER -