Detection of seven species of pathogenic leptospires by PCR using two sets of primers

Claudia Gravekamp, H. Van de Kemp, M. Franzen, D. Carrington, G. J. Schoone, G. J J M Van Eys, C. O R Everard, R. A. Hartskeerl, W. J. Terpstra

Research output: Contribution to journalArticle

222 Citations (Scopus)

Abstract

Two sets of primers derived from genomic DNA libraries of Leptospira serovars icterohaemorrhagiae (strain RGA) and bim (strain 1051) enabled the amplification by PCR of target DNA fragments from leptospiral reference strains belonging to all presently described pathogenic Leptospira species. The icterohaemorrhagiae-derived primers (G1/G2) enabled amplification of DNA from L. interrogans, L. borgpetersenii, L. weilii, L. noguchii, L. santarosai and L. meyeri, whereas the bim-derived primers (B64-I/B64-II) enabled the amplification of L. kirschneri. Southern blot and DNA sequence analysis revealed inter-species DNA polymorphism within the region spanned by primers G1 and G2 between L. interrogans and various other Leptospira species. Using a mixture of primer sets G1/G2 and B64-I/B64-II, leptospires of serovars icterohaemorrhagiae, copenhageni, hardjo, pomona, grippotyphosa and bim were detected in serum samples collected from patients during the first 10 days after the onset of illness.

Original languageEnglish (US)
Pages (from-to)1691-1700
Number of pages10
JournalJournal of General Microbiology
Volume139
Issue number8
StatePublished - 1993
Externally publishedYes

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Leptospira
Polymerase Chain Reaction
DNA
Genomic Library
Southern Blotting
Gene Library
DNA Sequence Analysis
Serum
Serogroup

ASJC Scopus subject areas

  • Microbiology

Cite this

Gravekamp, C., Van de Kemp, H., Franzen, M., Carrington, D., Schoone, G. J., Van Eys, G. J. J. M., ... Terpstra, W. J. (1993). Detection of seven species of pathogenic leptospires by PCR using two sets of primers. Journal of General Microbiology, 139(8), 1691-1700.

Detection of seven species of pathogenic leptospires by PCR using two sets of primers. / Gravekamp, Claudia; Van de Kemp, H.; Franzen, M.; Carrington, D.; Schoone, G. J.; Van Eys, G. J J M; Everard, C. O R; Hartskeerl, R. A.; Terpstra, W. J.

In: Journal of General Microbiology, Vol. 139, No. 8, 1993, p. 1691-1700.

Research output: Contribution to journalArticle

Gravekamp, C, Van de Kemp, H, Franzen, M, Carrington, D, Schoone, GJ, Van Eys, GJJM, Everard, COR, Hartskeerl, RA & Terpstra, WJ 1993, 'Detection of seven species of pathogenic leptospires by PCR using two sets of primers', Journal of General Microbiology, vol. 139, no. 8, pp. 1691-1700.
Gravekamp C, Van de Kemp H, Franzen M, Carrington D, Schoone GJ, Van Eys GJJM et al. Detection of seven species of pathogenic leptospires by PCR using two sets of primers. Journal of General Microbiology. 1993;139(8):1691-1700.
Gravekamp, Claudia ; Van de Kemp, H. ; Franzen, M. ; Carrington, D. ; Schoone, G. J. ; Van Eys, G. J J M ; Everard, C. O R ; Hartskeerl, R. A. ; Terpstra, W. J. / Detection of seven species of pathogenic leptospires by PCR using two sets of primers. In: Journal of General Microbiology. 1993 ; Vol. 139, No. 8. pp. 1691-1700.
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