Detection of migrated allogeneic oligodendrocytes throughout the central nervous system of the galactocerebrosidase-deficient twitcher mouse

W. Huppes, C. J A De Groot, R. H. Ostendorf, J. G J Bauman, J. A. Gossen, V. Smit, Jan Vijg, C. D. Dijkstra

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Galactocerebrosidase-deficient oligodendrocytes of 'twitcher' (twi/twi) mice degenerate prematurely. Transplantation of normal bone marrow cells has been shown to alleviate symptoms and to prolong survival time. However, characteristic ataxia ('twitching') is not cured. In an attempt to improve further the condition of twitcher mice, allogeneic foetal liver cells were transplanted as a source of normal haemopoietic stem cells and supplemented with intracerebral transplantation of foetal brain cells. A reliable method was developed to detect donor-type cells in brain tissue. Bacteriophage λ. transgenic foetal mice were used as donors of both foetal liver and brain cells. Integrated copies of λ. DNA in donor cells were detected by in situ hybridization with biotinylated probes, which were then stained using streptavidin alkaline phosphatase. This technique was combined with immunohistochemistry to distinguish donor-type oligodendrocytes from macrophages. Immunoperoxidase staining with an antiserum to carbonic anhydrase-II produced dark perikarya of oligodendrocytes. The results demonstrated that local foetal brain cell grafts resulted in a wide dissemination of donor-type oligodendrocytes throughout the twitcher brain. The addition of a foetal brain cell graft to haemopoietic cell transplantation resulted in significantly prolonged survival of twitcher mice.

Original languageEnglish (US)
Pages (from-to)129-136
Number of pages8
JournalJournal of Neurocytology
Volume21
Issue number2
DOIs
StatePublished - Feb 1992
Externally publishedYes

Fingerprint

Galactosylceramidase
Oligodendroglia
Central Nervous System
Brain
Carbonic Anhydrase II
Transplants
Streptavidin
Liver
Cell Transplantation
Ataxia
Bone Marrow Transplantation
Bacteriophages
Transgenic Mice
In Situ Hybridization
Alkaline Phosphatase
Immune Sera
Stem Cells
Transplantation
Immunohistochemistry
Macrophages

ASJC Scopus subject areas

  • Neuroscience(all)
  • Histology
  • Anatomy
  • Cell Biology

Cite this

Huppes, W., De Groot, C. J. A., Ostendorf, R. H., Bauman, J. G. J., Gossen, J. A., Smit, V., ... Dijkstra, C. D. (1992). Detection of migrated allogeneic oligodendrocytes throughout the central nervous system of the galactocerebrosidase-deficient twitcher mouse. Journal of Neurocytology, 21(2), 129-136. https://doi.org/10.1007/BF01189011

Detection of migrated allogeneic oligodendrocytes throughout the central nervous system of the galactocerebrosidase-deficient twitcher mouse. / Huppes, W.; De Groot, C. J A; Ostendorf, R. H.; Bauman, J. G J; Gossen, J. A.; Smit, V.; Vijg, Jan; Dijkstra, C. D.

In: Journal of Neurocytology, Vol. 21, No. 2, 02.1992, p. 129-136.

Research output: Contribution to journalArticle

Huppes, W. ; De Groot, C. J A ; Ostendorf, R. H. ; Bauman, J. G J ; Gossen, J. A. ; Smit, V. ; Vijg, Jan ; Dijkstra, C. D. / Detection of migrated allogeneic oligodendrocytes throughout the central nervous system of the galactocerebrosidase-deficient twitcher mouse. In: Journal of Neurocytology. 1992 ; Vol. 21, No. 2. pp. 129-136.
@article{d4f45fba319d4104b8b1c3f2ab6c318a,
title = "Detection of migrated allogeneic oligodendrocytes throughout the central nervous system of the galactocerebrosidase-deficient twitcher mouse",
abstract = "Galactocerebrosidase-deficient oligodendrocytes of 'twitcher' (twi/twi) mice degenerate prematurely. Transplantation of normal bone marrow cells has been shown to alleviate symptoms and to prolong survival time. However, characteristic ataxia ('twitching') is not cured. In an attempt to improve further the condition of twitcher mice, allogeneic foetal liver cells were transplanted as a source of normal haemopoietic stem cells and supplemented with intracerebral transplantation of foetal brain cells. A reliable method was developed to detect donor-type cells in brain tissue. Bacteriophage λ. transgenic foetal mice were used as donors of both foetal liver and brain cells. Integrated copies of λ. DNA in donor cells were detected by in situ hybridization with biotinylated probes, which were then stained using streptavidin alkaline phosphatase. This technique was combined with immunohistochemistry to distinguish donor-type oligodendrocytes from macrophages. Immunoperoxidase staining with an antiserum to carbonic anhydrase-II produced dark perikarya of oligodendrocytes. The results demonstrated that local foetal brain cell grafts resulted in a wide dissemination of donor-type oligodendrocytes throughout the twitcher brain. The addition of a foetal brain cell graft to haemopoietic cell transplantation resulted in significantly prolonged survival of twitcher mice.",
author = "W. Huppes and {De Groot}, {C. J A} and Ostendorf, {R. H.} and Bauman, {J. G J} and Gossen, {J. A.} and V. Smit and Jan Vijg and Dijkstra, {C. D.}",
year = "1992",
month = "2",
doi = "10.1007/BF01189011",
language = "English (US)",
volume = "21",
pages = "129--136",
journal = "Journal of Neurocytology",
issn = "0300-4864",
publisher = "Kluwer Academic Publishers",
number = "2",

}

TY - JOUR

T1 - Detection of migrated allogeneic oligodendrocytes throughout the central nervous system of the galactocerebrosidase-deficient twitcher mouse

AU - Huppes, W.

AU - De Groot, C. J A

AU - Ostendorf, R. H.

AU - Bauman, J. G J

AU - Gossen, J. A.

AU - Smit, V.

AU - Vijg, Jan

AU - Dijkstra, C. D.

PY - 1992/2

Y1 - 1992/2

N2 - Galactocerebrosidase-deficient oligodendrocytes of 'twitcher' (twi/twi) mice degenerate prematurely. Transplantation of normal bone marrow cells has been shown to alleviate symptoms and to prolong survival time. However, characteristic ataxia ('twitching') is not cured. In an attempt to improve further the condition of twitcher mice, allogeneic foetal liver cells were transplanted as a source of normal haemopoietic stem cells and supplemented with intracerebral transplantation of foetal brain cells. A reliable method was developed to detect donor-type cells in brain tissue. Bacteriophage λ. transgenic foetal mice were used as donors of both foetal liver and brain cells. Integrated copies of λ. DNA in donor cells were detected by in situ hybridization with biotinylated probes, which were then stained using streptavidin alkaline phosphatase. This technique was combined with immunohistochemistry to distinguish donor-type oligodendrocytes from macrophages. Immunoperoxidase staining with an antiserum to carbonic anhydrase-II produced dark perikarya of oligodendrocytes. The results demonstrated that local foetal brain cell grafts resulted in a wide dissemination of donor-type oligodendrocytes throughout the twitcher brain. The addition of a foetal brain cell graft to haemopoietic cell transplantation resulted in significantly prolonged survival of twitcher mice.

AB - Galactocerebrosidase-deficient oligodendrocytes of 'twitcher' (twi/twi) mice degenerate prematurely. Transplantation of normal bone marrow cells has been shown to alleviate symptoms and to prolong survival time. However, characteristic ataxia ('twitching') is not cured. In an attempt to improve further the condition of twitcher mice, allogeneic foetal liver cells were transplanted as a source of normal haemopoietic stem cells and supplemented with intracerebral transplantation of foetal brain cells. A reliable method was developed to detect donor-type cells in brain tissue. Bacteriophage λ. transgenic foetal mice were used as donors of both foetal liver and brain cells. Integrated copies of λ. DNA in donor cells were detected by in situ hybridization with biotinylated probes, which were then stained using streptavidin alkaline phosphatase. This technique was combined with immunohistochemistry to distinguish donor-type oligodendrocytes from macrophages. Immunoperoxidase staining with an antiserum to carbonic anhydrase-II produced dark perikarya of oligodendrocytes. The results demonstrated that local foetal brain cell grafts resulted in a wide dissemination of donor-type oligodendrocytes throughout the twitcher brain. The addition of a foetal brain cell graft to haemopoietic cell transplantation resulted in significantly prolonged survival of twitcher mice.

UR - http://www.scopus.com/inward/record.url?scp=0026558789&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026558789&partnerID=8YFLogxK

U2 - 10.1007/BF01189011

DO - 10.1007/BF01189011

M3 - Article

VL - 21

SP - 129

EP - 136

JO - Journal of Neurocytology

JF - Journal of Neurocytology

SN - 0300-4864

IS - 2

ER -