Detection of H2-b and Thy-1.2 surface antigens on the differentiating murine otocyst using Nomarski optics

Michael B. Prystowsky, K. M. Khan, W. F. Marovitz

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The 12th postcoital day otocyst appears as a hollow cellular ball of pseudostratified columnar epithelium that has entered into its initial stages of differentiation and organogenesis. H-2b antigen was demonstrated on the ectodermally derived epithelial cells of the otocysts and on the mesodermally derived cells of the surrounding mesenchyme. Thy-1.2 antigen was detected on the mesenchymal cells, but not on the epithelial cells of the otocyst. The use of Nomarski optics as a new method for detecting cell surface staining that would otherwise be undetected by bright field optics was demonstrated.

Original languageEnglish (US)
Pages (from-to)53-58
Number of pages6
JournalDifferentiation
Volume12
Issue number1
StatePublished - 1978
Externally publishedYes

Fingerprint

Thy-1 Antigens
Surface Antigens
Epithelial Cells
Organogenesis
Mesoderm
Epithelium
Staining and Labeling

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Pathology and Forensic Medicine

Cite this

Detection of H2-b and Thy-1.2 surface antigens on the differentiating murine otocyst using Nomarski optics. / Prystowsky, Michael B.; Khan, K. M.; Marovitz, W. F.

In: Differentiation, Vol. 12, No. 1, 1978, p. 53-58.

Research output: Contribution to journalArticle

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