Detection of H2-b and Thy-1.2 Surface Antigens on the Differentiating Murine Otocyst Using Nomarski Optics

MICHAEL B. PRYSTOWSKY, KHALID M. KHAN, WILLIAM F. MAROVITZ

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

The 12th postcoital day otocyst appears as a hollow cellular ball of pseudostratified columnar epithelium that has entered into its initial stages of differentiation and organogenesis. H-2b antigen was demonstrated on the ectodermally derived epithelial cells of the otocysts and on the mesodermally derived cells of the surrounding mesenchyme. Thy-1.2 antigen was detected on the mesenchymal cells, but not on the epithelial cells of the otocyst. The use of Nomarski optics as a new method for detecting cell surface staining that would otherwise be undetected by bright field optics was demonstrated.

Original languageEnglish (US)
Pages (from-to)53-58
Number of pages6
JournalDifferentiation
Volume12
Issue number1-3
DOIs
StatePublished - Jan 1 1979
Externally publishedYes

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology
  • Cancer Research

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