Design and validation of a dynamic cell-culture system for bone biology research and exogenous tissue-engineering applications

Alexander C. Allori, Edward H. Davidson, Derek D. Reformat, Alexander M. Sailon, James Freeman, Adam Vaughan, David Wootton, Elizabeth Clark, John L. Ricci, Stephen M. Warren

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Bone lacunocanalicular fluid flow ensures chemotransportation and provides a mechanical stimulus to cells. Traditional static cell-culture methods are ill-suited to study the intricacies of bone biology because they ignore the three-dimensionality of meaningful cellular networks and the lacunocanalicular system; furthermore, reliance on diffusion alone for nutrient supply and waste product removal effectively limits scaffolds to 2–3 mm thickness. In this project, a flow-perfusion system was custom-designed to overcome these limitations: eight adaptable chambers housed cylindrical cell-seeded scaffolds measuring 12 or 24 mm in diameter and 1–10 mm in thickness. The porous scaffolds were manufactured using a three-dimensional (3D) periodic microprinting process and were composed of hydroxyapatite/tricalcium phosphate with variable thicknesses, strut sizes, pore sizes and structural configurations. A multi-channel peristaltic pump drew medium from parallel reservoirs and perfused it through each scaffold at a programmable rate. Hermetically sealed valves permitted sampling or replacement of medium. A gas-permeable membrane allowed for gas exchange. Tubing was selected to withstand continuous perfusion for > 2 months without leakage. Computational modelling was performed to assess the adequacy of oxygen supply and the range of fluid shear stress in the bioreactor–scaffold system, using 12 × 6 mm scaffolds, and these models suggested scaffold design modifications that improved oxygen delivery while enhancing physiological shear stress. This system may prove useful in studying complex 3D bone biology and in developing strategies for engineering thick 3D bone constructs.

Original languageEnglish (US)
Pages (from-to)E327-E336
JournalJournal of Tissue Engineering and Regenerative Medicine
Volume10
Issue number10
DOIs
StatePublished - Oct 1 2016
Externally publishedYes

Fingerprint

Systems Biology
Tissue Engineering
Tissue engineering
Cell culture
Scaffolds
Bone
Cell Culture Techniques
Bone and Bones
Research
Perfusion
Gases
Shear stress
Oxygen
Waste Products
Gas permeable membranes
Oxygen supply
Durapatite
Struts
Tubing
Scaffolds (biology)

Keywords

  • bioreactor
  • bone
  • cell culture
  • fluid shear stress
  • lacunocanalicular system
  • scaffold
  • tissue engineering

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biomaterials
  • Biomedical Engineering

Cite this

Design and validation of a dynamic cell-culture system for bone biology research and exogenous tissue-engineering applications. / Allori, Alexander C.; Davidson, Edward H.; Reformat, Derek D.; Sailon, Alexander M.; Freeman, James; Vaughan, Adam; Wootton, David; Clark, Elizabeth; Ricci, John L.; Warren, Stephen M.

In: Journal of Tissue Engineering and Regenerative Medicine, Vol. 10, No. 10, 01.10.2016, p. E327-E336.

Research output: Contribution to journalArticle

Allori, Alexander C. ; Davidson, Edward H. ; Reformat, Derek D. ; Sailon, Alexander M. ; Freeman, James ; Vaughan, Adam ; Wootton, David ; Clark, Elizabeth ; Ricci, John L. ; Warren, Stephen M. / Design and validation of a dynamic cell-culture system for bone biology research and exogenous tissue-engineering applications. In: Journal of Tissue Engineering and Regenerative Medicine. 2016 ; Vol. 10, No. 10. pp. E327-E336.
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