TY - JOUR
T1 - Design and Initial Characterization of a Small Near-Infrared Fluorescent Calcium Indicator
AU - Matlashov, Mikhail E.
AU - Vera, Jorge
AU - Kasatkina, Ludmila A.
AU - Khodakhah, Kamran
AU - Verkhusha, Vladislav V.
N1 - Funding Information:
This work was supported by the grants R35 GM122567 and RF1 NS115581 from the U.S. National Institutes of Health, 226178 from the Chan Zuckerberg Initiative, and 322226 from the Academy of Finland.
Publisher Copyright:
Copyright © 2022 Matlashov, Vera, Kasatkina, Khodakhah and Verkhusha.
PY - 2022/6/29
Y1 - 2022/6/29
N2 - Near-infrared (NIR) genetically encoded calcium indicators (GECIs) are becoming powerful tools for neuroscience. Because of their spectral characteristics, the use of NIR GECIs helps to avoid signal loss from the absorption by body pigments, light-scattering, and autofluorescence in mammalian tissues. In addition, NIR GECIs do not suffer from cross-excitation artifacts when used with common fluorescent indicators and optogenetics actuators. Although several NIR GECIs have been developed, there is no NIR GECI currently available that would combine the high brightness in cells and photostability with small size and fast response kinetics. Here, we report a small FRET-based NIR fluorescent calcium indicator iGECInano. We characterize iGECInano in vitro, in non-neuronal mammalian cells, and primary mouse neurons. iGECInano demonstrates the improvement in the signal-to-noise ratio and response kinetics compared to other NIR GECIs.
AB - Near-infrared (NIR) genetically encoded calcium indicators (GECIs) are becoming powerful tools for neuroscience. Because of their spectral characteristics, the use of NIR GECIs helps to avoid signal loss from the absorption by body pigments, light-scattering, and autofluorescence in mammalian tissues. In addition, NIR GECIs do not suffer from cross-excitation artifacts when used with common fluorescent indicators and optogenetics actuators. Although several NIR GECIs have been developed, there is no NIR GECI currently available that would combine the high brightness in cells and photostability with small size and fast response kinetics. Here, we report a small FRET-based NIR fluorescent calcium indicator iGECInano. We characterize iGECInano in vitro, in non-neuronal mammalian cells, and primary mouse neurons. iGECInano demonstrates the improvement in the signal-to-noise ratio and response kinetics compared to other NIR GECIs.
KW - biosensor
KW - calcium imaging
KW - far-red
KW - FRET
KW - GECI
KW - iRFP
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U2 - 10.3389/fcell.2022.880107
DO - 10.3389/fcell.2022.880107
M3 - Article
AN - SCOPUS:85134157144
VL - 10
JO - Frontiers in Cell and Developmental Biology
JF - Frontiers in Cell and Developmental Biology
SN - 2296-634X
M1 - 880107
ER -