Derivation of a triple mosaic adenovirus based on modification of the minor capsid protein IX

Yizhe Tang, Long P. Le, Qiana L. Matthews, Tie Han, Hongju Wu, David T. Curiel

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Adenoviral capsid protein IX (pIX) has been shown to be a potential locale to insert targeting, imaging-related and therapeutic modalities by genetic modification. Recent evidences suggested that capsid protein mosaicism could be a promising strategy for improving the utility of Ad vector. In this study, we explored a method to genetically generate triple pIX mosaic Ad serotype 5 (Ad5) displaying three types of pIX on a single virion. pIXs were modified at their carboxy termini with a Flag sequence, a hexahistidine sequence (His6) or a monomeric red fluorescent protein (mRFP1), respectively. Western blotting analysis and fluorescence microscopy of the purified recombinant viruses indicated that all three modified pIXs were incorporated into the viral particles. Immuno-gold electron microscopy (EM) further confirmed that three types of pIX indeed co-existed on an individual virion. These results firstly validated a triple mosaic capsid configuration on pIX, and demonstrated the possibility of further radical design.

Original languageEnglish (US)
Pages (from-to)391-400
Number of pages10
JournalVirology
Volume377
Issue number2
DOIs
Publication statusPublished - Aug 1 2008

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Keywords

  • Adenovirus
  • Triple mosaic Ad
  • pIX modification

ASJC Scopus subject areas

  • Virology

Cite this

Tang, Y., Le, L. P., Matthews, Q. L., Han, T., Wu, H., & Curiel, D. T. (2008). Derivation of a triple mosaic adenovirus based on modification of the minor capsid protein IX. Virology, 377(2), 391-400. https://doi.org/10.1016/j.virol.2008.04.023