Demodex musculi infestation in genetically immunomodulated mice

Peter C. Smith, Caroline J. Zeiss, Amanda P. Beck, Jodi A. Scholz

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Demodex musculi, a prostigmatid mite that has been reported infrequently in laboratory mice, has been identified with increasing frequency in contemporary colonies of immunodeficient mice. Here we describe 2 episodes of D. musculi infestation with associated clinical signs in various genetically engineered mouse strains, as well as treatment strategies and an investigation into transmissibility and host susceptibility. The first case involved D. musculi associated with clinical signs and pathologic lesions in BALB/c-Tg(DO11.10)Il13tm mice, which have a defect in type 2 helper T cell (Th2) immunity. Subsequent investigation revealed mite transmission to both parental strains (BALB/c-Tg[DO11.10] and BALB/c-Il13tm), BALB/c-Il13/Il4tm, and wild-type BALB/c. All Tg(DO11.10)Il13tm mice remained infested throughout the investigation, and D. musculi were recovered from all strains when they were cohoused with BALB/c-Tg(DO11.10)Il13tm index mice. However, only Il13tm and Il13/Il4tm mice demonstrated persistent infestation after index mice were removed. Only BALB/c-Tg(DO11.10)Il13tm showed clinical signs, suggesting that the phenotypic dysfunction of Th2 immunity is sufficient for persistent infestation, whereas clinical disease associated with D. musculi appears to be genotype-specific. This pattern was further exemplified in the second case, which involved NOD.Cg-PrkdcscidIl2rgtm1Wjl/SzJ (NSG) and C;129S4 Rag2tm1.1Flv Il2rgtm1.1Flv/J mice with varying degrees of blepharitis, conjunctivitis, and facial pruritis. Topical amitraz decreased mite burden but did not eliminate infestation or markedly ameliorate clinical signs. Furthermore, mite burden began to increase by 1 mo posttreatment, suggesting that topical amitraz is an ineffective treatment for D. musculi. These experiences illustrate the need for vigilance regarding opportunistic and uncommon pathogens in rodent colonies, especially among mice with immunologic deficits.

Original languageEnglish (US)
Pages (from-to)278-285
Number of pages8
JournalComparative Medicine
Volume66
Issue number4
StatePublished - Aug 1 2016
Externally publishedYes

Fingerprint

Demodex
Interleukin-13
mice
Mites
T-cells
Pathogens
mites
amitraz
Defects
Immunity
immunity
Blepharitis
Th2 Cells
conjunctivitis
Conjunctivitis
pruritus
Pruritus
lesions (animal)
Rodentia
rodents

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • veterinary(all)

Cite this

Smith, P. C., Zeiss, C. J., Beck, A. P., & Scholz, J. A. (2016). Demodex musculi infestation in genetically immunomodulated mice. Comparative Medicine, 66(4), 278-285.

Demodex musculi infestation in genetically immunomodulated mice. / Smith, Peter C.; Zeiss, Caroline J.; Beck, Amanda P.; Scholz, Jodi A.

In: Comparative Medicine, Vol. 66, No. 4, 01.08.2016, p. 278-285.

Research output: Contribution to journalArticle

Smith, PC, Zeiss, CJ, Beck, AP & Scholz, JA 2016, 'Demodex musculi infestation in genetically immunomodulated mice', Comparative Medicine, vol. 66, no. 4, pp. 278-285.
Smith, Peter C. ; Zeiss, Caroline J. ; Beck, Amanda P. ; Scholz, Jodi A. / Demodex musculi infestation in genetically immunomodulated mice. In: Comparative Medicine. 2016 ; Vol. 66, No. 4. pp. 278-285.
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