Deletions within the mouse β-globin locus control region preferentially reduce β(min) globin gene expression

Raouf Alami, M. A. Bender, Yong Qing Feng, Steven N. Fiering, Bruce A. Hug, Timothy J. Ley, Mark Groudine, Eric E. Bouhassira

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

The mouse β-globin gene cluster is regulated, at least in part, by a locus control region (LCR) composed of several developmentally stable DNase I hypersensitive sites located upstream of the genes. In this report, we examine the level of expression of the β(min) and β(maj) genes in adult mice in which HS2, HS3, or HS5,6 has been either deleted or replaced by a selectable marker via homologous recombination in ES cells. Primer extension analysis of RNA extracted from circulating reticulocytes and HPLC analysis of globin chains from peripheral red blood cells revealed that all mutations that reduce the overall output of the locus preferentially decrease β(min) expression over β(maj). The implications of these findings for the mechanism by which the LCR controls expression of the β(maj) and β(min) promoters are discussed. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)417-424
Number of pages8
JournalGenomics
Volume63
Issue number3
DOIs
StatePublished - Feb 1 2000

ASJC Scopus subject areas

  • Genetics

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    Alami, R., Bender, M. A., Feng, Y. Q., Fiering, S. N., Hug, B. A., Ley, T. J., Groudine, M., & Bouhassira, E. E. (2000). Deletions within the mouse β-globin locus control region preferentially reduce β(min) globin gene expression. Genomics, 63(3), 417-424. https://doi.org/10.1006/geno.1999.6104