Definition of a core region of RAG-2 that is functional in V(D)J recombination

Moshe J. Sadofsky, Joanne E. Hesse, Martin Gellert

Research output: Contribution to journalArticle

139 Citations (Scopus)

Abstract

The products of the RAG-1 and RAG-2 genes cooperate to allow V(D)J recombination in lymphoid and non-lymphoid cells. As one step toward understanding the role of RAG-2, we have constructed mutated RAG-2 genes and examined their ability to support recombination of plasmid substrates in a fibroblast cell line. The mutations define essential and dispensable parts of the RAG-2 gene. Mutations in the N-terminal part eliminate almost all activity. In the central region of the protein, some but not all local alterations still allow recombination. On the other hand, proteins with large deletions from the C-terminal end, including one truncated by 25%, still retain activity, even though this part of the protein is highly conserved between species. Similar results were obtained with substrates that retain either a signal joint or a coding joint, or perform an inversion. Thus all basic features of V(D)J joining are retained in a RAG-2 protein with only the first 75% of the sequence.

Original languageEnglish (US)
Pages (from-to)1805-1809
Number of pages5
JournalNucleic Acids Research
Volume22
Issue number10
StatePublished - May 25 1994
Externally publishedYes

Fingerprint

V(D)J Recombination
Recombination
Genes
Proteins
Protein
Genetic Recombination
RAG-1 Genes
Joints
Gene
Mutation
Substrate
Substrates
Fibroblasts
Joining
Cell
Plasmids
Cells
Deletion
Cell Line
Inversion

ASJC Scopus subject areas

  • Genetics
  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)

Cite this

Definition of a core region of RAG-2 that is functional in V(D)J recombination. / Sadofsky, Moshe J.; Hesse, Joanne E.; Gellert, Martin.

In: Nucleic Acids Research, Vol. 22, No. 10, 25.05.1994, p. 1805-1809.

Research output: Contribution to journalArticle

Sadofsky, Moshe J. ; Hesse, Joanne E. ; Gellert, Martin. / Definition of a core region of RAG-2 that is functional in V(D)J recombination. In: Nucleic Acids Research. 1994 ; Vol. 22, No. 10. pp. 1805-1809.
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