Deduction from Wilms' tumour that glomerular podocytes produce the basement membrane material bearing Goodpasture determinants

D. Droz, N. Diebold, Dominique M. Jan, F. Jaubert

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Using an indirect immunoperoxidase technique, we tested frozen specimens from one Wilms' tumour composed of numerous glomeruloid bodies devoid of blood vessels, with monoclonal antibodies directed against vimentin, cytokeratin, CALLA/CD10, CD24, CR1/CD35, endothelium factor VIII, class I and II MHC molecules, laminin, fibronectin, and non-collagenic domain NC1 of type IV collagen. Two reagents against Goodpasture determinants were used: P1 monoclonal antibody and serum IgG (GP antibodies) from a biopsy-proven Goodpasture patient. Glomeruloid bodies comprised two cell types: a peripheral layer of parietal epithelial cells (cytokeratin and CD24-positive) and central cell clumps of podocytes (vimentin and CALLA-positive). The basal lamina surrounding the glomeruloid bodies contained laminin and NCl domain of type IV collagen, while that present between the podocytes reacted strongly with laminin, and P1 and GP antibodies. Endothelium factor VIII was not detected within the glomeruloid bodies and CR1 molecules bound to the basement membrane material within them. These data favour the hypothesis that podocytes produce the basement membrane material which bears Goodpasture determinants recently identified as a novel chain, named the α3 chain, of type IV collagen.

Original languageEnglish (US)
Pages (from-to)323-327
Number of pages5
JournalJournal of Pathology
Volume162
Issue number4
DOIs
StatePublished - 1990
Externally publishedYes

Fingerprint

Podocytes
Collagen Type IV
Wilms Tumor
Basement Membrane
Factor VIII
Laminin
Vimentin
Keratins
Endothelium
Monoclonal Antibodies
Antibodies
Immunoenzyme Techniques
Fibronectins
Blood Vessels
Immunoglobulin G
Epithelial Cells
Biopsy
Serum

Keywords

  • glomerular basement membrane
  • glomerular podocytes
  • Goodpasture determinants
  • type IV collagen
  • Wilms' tumour

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Deduction from Wilms' tumour that glomerular podocytes produce the basement membrane material bearing Goodpasture determinants. / Droz, D.; Diebold, N.; Jan, Dominique M.; Jaubert, F.

In: Journal of Pathology, Vol. 162, No. 4, 1990, p. 323-327.

Research output: Contribution to journalArticle

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