Decreased insulin content and secretion in RIN 1046-38 cells overexpressing α 2-adrenergic receptors

Maria S. Rodriguez-Pena, Regina Collins, Charles Woodard, Allen M. Spiegel

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Several G(i)-protein-coupled receptors normally expressed in islet β- cells inhibit insulin secretion on binding of their respective agonists. To study the effect of supraphysiologic expression of such a receptor in insulin-secreting β-cells, we stably transfected cDNA encoding the mouse α(2a)-adrenergic receptor into RIN 1046-38 cells. Four different cell lines were selected, each overexpressing the α(2a)-adrenergic receptor to varying degrees. Cell lines showing the highest level of receptor expression showed significantly reduced insulin content, and reduced basal and stimulated insulin secretion. Pertussis toxin (PTX) treatment of cells was able to reverse partially the reduced insulin secretory response. Our results suggest that overexpression of a G(i)-protein-coupled receptor in β-cells causes tonic inhibition of both insulin synthesis and secretion. Abnormalities in expression or function of such receptors could be a contributory factor in the impaired insulin secretion present in type II diabetes.

Original languageEnglish (US)
Pages (from-to)255-260
Number of pages6
JournalEndocrine
Volume7
Issue number2
StatePublished - 1997
Externally publishedYes

Fingerprint

Adrenergic Receptors
Insulin
G-Protein-Coupled Receptors
Cell Line
Pertussis Toxin
Insulin-Secreting Cells
Islets of Langerhans
Type 2 Diabetes Mellitus
Complementary DNA

Keywords

  • α -adrenergic receptor
  • G-protein
  • RIN cells

ASJC Scopus subject areas

  • Endocrinology

Cite this

Decreased insulin content and secretion in RIN 1046-38 cells overexpressing α 2-adrenergic receptors. / Rodriguez-Pena, Maria S.; Collins, Regina; Woodard, Charles; Spiegel, Allen M.

In: Endocrine, Vol. 7, No. 2, 1997, p. 255-260.

Research output: Contribution to journalArticle

Rodriguez-Pena, Maria S. ; Collins, Regina ; Woodard, Charles ; Spiegel, Allen M. / Decreased insulin content and secretion in RIN 1046-38 cells overexpressing α 2-adrenergic receptors. In: Endocrine. 1997 ; Vol. 7, No. 2. pp. 255-260.
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