Data Descriptor: Viruses of the Nahant Collection, characterization of 251 marine Vibrionaceae viruses

Kathryn M. Kauffman, Julia M. Brown, Radhey S. Sharma, David VanInsberghe, Joseph Elsherbini, Martin Polz, Libusha Kelly

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

Viruses are highly discriminating in their interactions with host cells and are thought to play a major role in maintaining diversity of environmental microbes. However, large-scale ecological and genomic studies of co-occurring virus-host pairs, required to characterize the mechanistic and genomic foundations of virus-host interactions, are lacking. Here, we present the largest dataset of cultivated and sequenced co-occurring virus-host pairs that captures ecologically representative fine-scale diversity. Using the ubiquitous and ecologically diverse marine Vibrionaceae as a host platform, we isolate and sequence 251 dsDNA viruses and their hosts from three time points within a 93-day time-series study. The virus collection includes representatives of the three Caudovirales tailed virus morphotypes, a novel family of nontailed viruses, and the smallest (10,046 bp) and largest (348,911 bp) Vibrio virus genomes described. We provide general characterization and annotation of the viruses and describe read-mapping protocols to standardize genome presentation. The rich ecological and genomic contextualization of hosts and viruses make the Nahant Collection a unique platform for high-resolution studies of environmental virus-host infection networks.

Original languageEnglish (US)
Article number180114
JournalScientific Data
Volume5
DOIs
Publication statusPublished - Jul 3 2018

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ASJC Scopus subject areas

  • Statistics and Probability
  • Information Systems
  • Education
  • Computer Science Applications
  • Statistics, Probability and Uncertainty
  • Library and Information Sciences

Cite this

Kauffman, K. M., Brown, J. M., Sharma, R. S., VanInsberghe, D., Elsherbini, J., Polz, M., & Kelly, L. (2018). Data Descriptor: Viruses of the Nahant Collection, characterization of 251 marine Vibrionaceae viruses. Scientific Data, 5, [180114]. https://doi.org/10.1038/sdata.2018.114