Cytoplasmic recruitment of INI1 and PML on incoming HIV preintegration complexes: Interference with early steps of viral replication

Priscilla Turelli, Vassilis Doucas, Errol Craig, Bastien Mangeat, Natacha Klages, Ronald Evans, Ganjam Kalpana, Didier Trono

Research output: Contribution to journalArticle

192 Scopus citations


During the early phase of the retroviral life cycle, only a fraction of internalized virions end up integrating their genome into the chromosome, even though the resulting proviruses are almost systematically expressed. Here, we reveal that incoming retroviral preintegration complexes trigger the exportin-mediated cytoplasmic export of the SWI/SNF component INI1 and of the nuclear body constituent PML. We further show that the HIV genome associates with these proteins before nuclear migration. In the presence of arsenic, PML is sequestered in the nucleus, and the efficiency of HIV-mediated transduction is markedly increased. These results unveil a so far unsuspected cellular response that interferes with the early steps of HIV replication.

Original languageEnglish (US)
Pages (from-to)1245-1254
Number of pages10
JournalMolecular Cell
Issue number6
StatePublished - Jan 1 2001


ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this