Cytoplasmic recruitment of INI1 and PML on incoming HIV preintegration complexes: Interference with early steps of viral replication

Priscilla Turelli, Vassilis Doucas, Errol Craig, Bastien Mangeat, Natacha Klages, Ronald Evans, Ganjam V. Kalpana, Didier Trono

Research output: Contribution to journalArticle

189 Citations (Scopus)

Abstract

During the early phase of the retroviral life cycle, only a fraction of internalized virions end up integrating their genome into the chromosome, even though the resulting proviruses are almost systematically expressed. Here, we reveal that incoming retroviral preintegration complexes trigger the exportin-mediated cytoplasmic export of the SWI/SNF component INI1 and of the nuclear body constituent PML. We further show that the HIV genome associates with these proteins before nuclear migration. In the presence of arsenic, PML is sequestered in the nucleus, and the efficiency of HIV-mediated transduction is markedly increased. These results unveil a so far unsuspected cellular response that interferes with the early steps of HIV replication.

Original languageEnglish (US)
Pages (from-to)1245-1254
Number of pages10
JournalMolecular Cell
Volume7
Issue number6
DOIs
StatePublished - 2001

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HIV
Karyopherins
Genome
Proviruses
Arsenic
Nuclear Proteins
Life Cycle Stages
Virion
Chromosomes

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Cytoplasmic recruitment of INI1 and PML on incoming HIV preintegration complexes : Interference with early steps of viral replication. / Turelli, Priscilla; Doucas, Vassilis; Craig, Errol; Mangeat, Bastien; Klages, Natacha; Evans, Ronald; Kalpana, Ganjam V.; Trono, Didier.

In: Molecular Cell, Vol. 7, No. 6, 2001, p. 1245-1254.

Research output: Contribution to journalArticle

Turelli, Priscilla ; Doucas, Vassilis ; Craig, Errol ; Mangeat, Bastien ; Klages, Natacha ; Evans, Ronald ; Kalpana, Ganjam V. ; Trono, Didier. / Cytoplasmic recruitment of INI1 and PML on incoming HIV preintegration complexes : Interference with early steps of viral replication. In: Molecular Cell. 2001 ; Vol. 7, No. 6. pp. 1245-1254.
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