Cytoplasmic dynein regulates its attachment to microtubules via nucleotide state-switched mechanosensing at multiple AAA domains

Matthew P. Nicholas, Florian Berger, Lu Rao, Sibylle Brenner, Carol Cho, Arne Gennerich

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Cytoplasmic dynein is a homodimeric microtubule (MT) motor protein responsible for most MT minus-end-directed motility. Dynein contains four AAA+ ATPases (AAA: ATPase associated with various cellular activities) per motor domain (AAA1-4). The main site of ATP hydrolysis, AAA1, is the only site considered by most dynein motility models. However, it remains unclear how ATPase activity and MT binding are coordinated within and between dynein's motor domains. Using optical tweezers, we characterize the MT-binding strength of recombinant dynein monomers as a function of mechanical tension and nucleotide state. Dynein responds anisotropically to tension, binding tighter to MTs when pulled toward the MT plus end. We provide evidence that this behavior results from an asymmetrical bond that acts as a slip bond under forward tension and a slip-ideal bond under backward tension. ATP weakens MT binding and reduces bond strength anisotropy, and unexpectedly, so does ADP. Using nucleotide binding and hydrolysis mutants, we show that, although ATP exerts its effects via binding AAA1, ADP effects are mediated by AAA3. Finally, we demonstrate "gating" of AAA1 function by AAA3. When tension is absent or applied via dynein's C terminus, ATP binding to AAA1 induces MT release only if AAA3 is in the posthydrolysis state. However, when tension is applied to the linker, ATP binding to AAA3 is sufficient to "open" the gate. These results elucidate the mechanisms of dynein - MT interactions, identify regulatory roles for AAA3, and help define the interplay between mechanical tension and nucleotide state in regulating dynein motility.

Original languageEnglish (US)
Pages (from-to)6371-6376
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume112
Issue number20
DOIs
StatePublished - May 19 2015

Fingerprint

Cytoplasmic Dyneins
Dyneins
Microtubules
Nucleotides
Adenosine Triphosphate
Adenosine Triphosphatases
Adenosine Diphosphate
Hydrolysis
Optical Tweezers
Microtubule Proteins
Anisotropy
Motor Activity

Keywords

  • AAA+ ATPases
  • Cytoplasmic dynein
  • Mechanosensing
  • Microtubules
  • Optical tweezers

ASJC Scopus subject areas

  • General

Cite this

Cytoplasmic dynein regulates its attachment to microtubules via nucleotide state-switched mechanosensing at multiple AAA domains. / Nicholas, Matthew P.; Berger, Florian; Rao, Lu; Brenner, Sibylle; Cho, Carol; Gennerich, Arne.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 112, No. 20, 19.05.2015, p. 6371-6376.

Research output: Contribution to journalArticle

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