Cytokine gene expression of endothelial cells infected with trypanosoma cruzi

Herbert B. Tanowitz, Jeff P. Gumprecht, Douglas Spurr, Tina M. Calderon, Maria C. Ventura, Carmen Raventos-Suarez, Susan Kellie, Stephen M. Factor, Victor B. Hatcher, Murray Wittner, Joan W. Berman

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56 Scopus citations

Abstract

Coronary microvascular spasm and platelet hyperreactivity have been implicated in the pathogenesis of Chagas’ cardiomyopathy. To clarify further the role of the microvasculature in this disease, alterations in cytokine gene expression due to Trypanosoma cruzi infection of human umbilical vein endothelial cells were examined. Northern blot analysis of total RNA from endothelial cells demonstrated that interleukin (IL)-1β, IL-6, and colony-stimulating factor 1 (CSF-1) mRNA expression was absent or minimal in uninfected cells but significantly increased in infected cells, c-sis mRNA levels diminished with increased time of infection. In situ hybridization studies also demonstrated high levels of IL-6 mRNA in individual infected cells. Significant levels of IL-6 and IL-1β protein were detected in the supernatants of infected endothelial cells. The serum of an acutely infected individual contained high levels of IL-6 protein, suggesting the potential importance of cytokines secreted by the vascular endothelium in the pathogenesis of Chagas’ cardiomyopathy.

Original languageEnglish (US)
Pages (from-to)598-603
Number of pages6
JournalJournal of Infectious Diseases
Volume166
Issue number3
DOIs
StatePublished - Sep 1992

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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    Tanowitz, H. B., Gumprecht, J. P., Spurr, D., Calderon, T. M., Ventura, M. C., Raventos-Suarez, C., Kellie, S., Factor, S. M., Hatcher, V. B., Wittner, M., & Berman, J. W. (1992). Cytokine gene expression of endothelial cells infected with trypanosoma cruzi. Journal of Infectious Diseases, 166(3), 598-603. https://doi.org/10.1093/infdis/166.3.598