Cyclin D1 regulates cellular migration through the inhibition of thrombospondin 1 and ROCK signaling

Zhiping Li, Chenguang Wang, Xuanmao Jiao, Yinan Lu, Maofu Fu, Andrew A. Quong, Chip Dye, Jianguo Yang, Maozheng Dai, Xiaoming Ju, Xueping Zhang, Anping Li, Peter Burbelo, E. Richard Stanley, Richard G. Pestell

Research output: Contribution to journalArticle

127 Citations (Scopus)

Abstract

Cyclin D1 is overexpressed in human tumors, correlating with cellular metastasis, and is induced by activating Rho GTPases. Herein, cyclin D1-deficient mouse embryo fibroblasts (MEFs) exhibited increased adhesion and decreased motility compared with wild-type MEFs. Retroviral transduction of cyclin D1 reversed these phenotypes. Mutational analysis of cyclin D1 demonstrated that its effects on cellular adhesion and migration were independent of the pRb and p160 coactivator binding domains. Genomewide expression arrays identified a subset of genes regulated by cyclin D1, including Rho-activated kinase II (ROCKII) and thrombospondin 1 (TSP-1). cyclin D1 -/- cells showed increased Rho GTP and ROCKII activity and signaling, with increased phosphorylation of LIM kinase, cofilin (Ser3), and myosin light chain 2 (Thr18/Ser19). Cyclin D1 repressed ROCKII and TSP-1 expression, and the migratory defect of cyclin D1-/- cells was reversed by ROCK inhibition or TSP-1 immunoneutralizing antibodies, cyclin E knockin to the cyclin D1-/- MEFs rescued the DNA synthesis defect of cyclin D1 -/- MEFs but did not rescue either the migration defect or the abundance of ROCKII. Cyclin D1 promotes cellular motility through inhibiting ROCK signaling and repressing the metastasis suppressor TSP-1.

Original languageEnglish (US)
Pages (from-to)4240-4256
Number of pages17
JournalMolecular and Cellular Biology
Volume26
Issue number11
DOIs
StatePublished - Jun 2006

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Thrombospondin 1
Cyclin D1
rho-Associated Kinases
Embryonic Structures
Fibroblasts
Lim Kinases
bcl-1 Genes
Actin Depolymerizing Factors
Neoplasm Metastasis
Cyclin E
rho GTP-Binding Proteins
Guanosine Triphosphate
Phosphorylation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Cyclin D1 regulates cellular migration through the inhibition of thrombospondin 1 and ROCK signaling. / Li, Zhiping; Wang, Chenguang; Jiao, Xuanmao; Lu, Yinan; Fu, Maofu; Quong, Andrew A.; Dye, Chip; Yang, Jianguo; Dai, Maozheng; Ju, Xiaoming; Zhang, Xueping; Li, Anping; Burbelo, Peter; Stanley, E. Richard; Pestell, Richard G.

In: Molecular and Cellular Biology, Vol. 26, No. 11, 06.2006, p. 4240-4256.

Research output: Contribution to journalArticle

Li, Z, Wang, C, Jiao, X, Lu, Y, Fu, M, Quong, AA, Dye, C, Yang, J, Dai, M, Ju, X, Zhang, X, Li, A, Burbelo, P, Stanley, ER & Pestell, RG 2006, 'Cyclin D1 regulates cellular migration through the inhibition of thrombospondin 1 and ROCK signaling', Molecular and Cellular Biology, vol. 26, no. 11, pp. 4240-4256. https://doi.org/10.1128/MCB.02124-05
Li, Zhiping ; Wang, Chenguang ; Jiao, Xuanmao ; Lu, Yinan ; Fu, Maofu ; Quong, Andrew A. ; Dye, Chip ; Yang, Jianguo ; Dai, Maozheng ; Ju, Xiaoming ; Zhang, Xueping ; Li, Anping ; Burbelo, Peter ; Stanley, E. Richard ; Pestell, Richard G. / Cyclin D1 regulates cellular migration through the inhibition of thrombospondin 1 and ROCK signaling. In: Molecular and Cellular Biology. 2006 ; Vol. 26, No. 11. pp. 4240-4256.
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