Cyclin A2 induces cardiac regeneration after myocardial infarction through cytokinesis of adult cardiomyocytes

Scott D. Shapiro, Amaresh K. Ranjan, Yoshiaki Kawase, Richard K. Cheng, Rina J. Kara, Romit Bhattacharya, Gabriela Guzman-Martinez, Javier Sanz, Mario J. Garcia, Hina W. Chaudhry

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Abstract

Cyclin A2 (Ccna2), normally silenced after birth in the mammalian heart, can induce cardiac repair in small-animal models of myocardial infarction. We report that delivery of the Ccna2 gene to infarcted porcine hearts invokes a regenerative response. We used a catheter-based approach to occlude the left anterior descending artery in swine, which resulted in substantial myocardial infarction. A week later, we performed left lateral thoracotomy and injected adenovirus carrying complementary DNA encoding CCNA2 or null adenovirus into peri-infarct myocardium. Six weeks after treatment, we assessed cardiac contractile function using multimodality imaging including magnetic resonance imaging, which demonstrated ∼18% increase in ejection fraction of Ccna2-treated pigs and ∼4% decrease in control pigs. Histologic studies demonstrate in vivo evidence of increased cardiomyocyte mitoses, increased cardiomyocyte number, and decreased fibrosis in the experimental pigs. Using time-lapse microscopic imaging of cultured adult porcine cardiomyocytes, we also show that Ccna2 elicits cytokinesis of adult porcine cardiomyocytes with preservation of sarcomeric structure. These data provide a compelling framework for the design and development of cardiac regenerative therapies based on cardiomyocyte cell cycle regulation.

Original languageEnglish (US)
Article number224ra27
JournalScience Translational Medicine
Volume6
Issue number224
DOIs
StatePublished - Feb 19 2014

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Cyclin A2
Cytokinesis
Cardiac Myocytes
Regeneration
Swine
Myocardial Infarction
Adenoviridae
Time-Lapse Imaging
Thoracotomy
Mitosis
Myocardium
Cell Cycle
Fibrosis
Catheters
Animal Models
Complementary DNA
Arteries
Magnetic Resonance Imaging
Parturition

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Shapiro, S. D., Ranjan, A. K., Kawase, Y., Cheng, R. K., Kara, R. J., Bhattacharya, R., ... Chaudhry, H. W. (2014). Cyclin A2 induces cardiac regeneration after myocardial infarction through cytokinesis of adult cardiomyocytes. Science Translational Medicine, 6(224), [224ra27]. https://doi.org/10.1126/scitranslmed.3007668

Cyclin A2 induces cardiac regeneration after myocardial infarction through cytokinesis of adult cardiomyocytes. / Shapiro, Scott D.; Ranjan, Amaresh K.; Kawase, Yoshiaki; Cheng, Richard K.; Kara, Rina J.; Bhattacharya, Romit; Guzman-Martinez, Gabriela; Sanz, Javier; Garcia, Mario J.; Chaudhry, Hina W.

In: Science Translational Medicine, Vol. 6, No. 224, 224ra27, 19.02.2014.

Research output: Contribution to journalArticle

Shapiro, SD, Ranjan, AK, Kawase, Y, Cheng, RK, Kara, RJ, Bhattacharya, R, Guzman-Martinez, G, Sanz, J, Garcia, MJ & Chaudhry, HW 2014, 'Cyclin A2 induces cardiac regeneration after myocardial infarction through cytokinesis of adult cardiomyocytes', Science Translational Medicine, vol. 6, no. 224, 224ra27. https://doi.org/10.1126/scitranslmed.3007668
Shapiro, Scott D. ; Ranjan, Amaresh K. ; Kawase, Yoshiaki ; Cheng, Richard K. ; Kara, Rina J. ; Bhattacharya, Romit ; Guzman-Martinez, Gabriela ; Sanz, Javier ; Garcia, Mario J. ; Chaudhry, Hina W. / Cyclin A2 induces cardiac regeneration after myocardial infarction through cytokinesis of adult cardiomyocytes. In: Science Translational Medicine. 2014 ; Vol. 6, No. 224.
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