Cycle-dependent matrix remodeling gene expression response in fatigue-loaded rat patellar tendons

Hui (Herb) Sun, Nelly Andarawis-Puri, Yonghui Li, David T. Fung, Jonathan Y. Lee, Vincent M. Wang, Jelena Basta-Pljakic, Daniel J. Leong, Jedd B. Sereysky, Stephen J. Ros, Raymond A. Klug, Jonathan Braman, Mitch B. Schaffler, Karl J. Jepsen, Evan L. Flatow

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Expression profiling of selected matrix remodeling genes was conducted to evaluate differences in molecular response to lowcycle (100) and high-cycle (7,200) sub-failure-fatigue loading of patellar tendons. Using our previously developed in vivo patellar tendon model, tendons were loaded for 100 or 7,200 cycles and expression of selected metalloproteinases (MMPs), tissue inhibitors of metalloproteinases (TIMPs), and collagens were quantified by real-time RT-PCR at 1- and 7-day post-loading. Expression profiles were also obtained from lacerated tendons as an acute injury model. The high-cycle group showed upregulation of TIMP-1, -2, Col3a1, and Col5a1, and downregulation TIMP-4 at both time points, upregulation of MMP-2 at 7-day post-loading and downregulation of MMP-13 and -14 at 1-day post-loading, suggesting overall repair/remodeling. In contrast, the low-cycle loaded group showed upregulation of MMP-2, -3, -13, and Col12a1 at both time points, upregulation of TIMP-1, -2, -3, Col3a1, and integrin b1 and downregulation of integrin a11 at 1-day post-loading and upregulation of Col1a1 at 7-day post-loading, consistent with a hypertrophic (adaptive) pattern. Lacerated tendons showed a typical acute wound response with upregulation of all examined remodeling genes. Differences found in tendon response to high- and low-cycle loading are suggestive of the underlying mechanisms associated with a healthy or damaging response.

Original languageEnglish (US)
Pages (from-to)1380-1386
Number of pages7
JournalJournal of Orthopaedic Research
Volume28
Issue number10
DOIs
StatePublished - 2010
Externally publishedYes

Fingerprint

Patellar Ligament
Fatigue
Up-Regulation
Gene Expression
Tendons
Matrix Metalloproteinases
Tissue Inhibitor of Metalloproteinase-2
Tissue Inhibitor of Metalloproteinase-1
Down-Regulation
Integrins
Tissue Inhibitor of Metalloproteinases
Matrix Metalloproteinase Inhibitors
Wounds and Injuries
Metalloproteases
Genes
Real-Time Polymerase Chain Reaction
Collagen

Keywords

  • Collagen
  • Fatigue loading
  • MMP
  • Patellar tendon
  • TIMP

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Medicine(all)

Cite this

Sun, H. H., Andarawis-Puri, N., Li, Y., Fung, D. T., Lee, J. Y., Wang, V. M., ... Flatow, E. L. (2010). Cycle-dependent matrix remodeling gene expression response in fatigue-loaded rat patellar tendons. Journal of Orthopaedic Research, 28(10), 1380-1386. https://doi.org/10.1002/jor.21132

Cycle-dependent matrix remodeling gene expression response in fatigue-loaded rat patellar tendons. / Sun, Hui (Herb); Andarawis-Puri, Nelly; Li, Yonghui; Fung, David T.; Lee, Jonathan Y.; Wang, Vincent M.; Basta-Pljakic, Jelena; Leong, Daniel J.; Sereysky, Jedd B.; Ros, Stephen J.; Klug, Raymond A.; Braman, Jonathan; Schaffler, Mitch B.; Jepsen, Karl J.; Flatow, Evan L.

In: Journal of Orthopaedic Research, Vol. 28, No. 10, 2010, p. 1380-1386.

Research output: Contribution to journalArticle

Sun, HH, Andarawis-Puri, N, Li, Y, Fung, DT, Lee, JY, Wang, VM, Basta-Pljakic, J, Leong, DJ, Sereysky, JB, Ros, SJ, Klug, RA, Braman, J, Schaffler, MB, Jepsen, KJ & Flatow, EL 2010, 'Cycle-dependent matrix remodeling gene expression response in fatigue-loaded rat patellar tendons', Journal of Orthopaedic Research, vol. 28, no. 10, pp. 1380-1386. https://doi.org/10.1002/jor.21132
Sun, Hui (Herb) ; Andarawis-Puri, Nelly ; Li, Yonghui ; Fung, David T. ; Lee, Jonathan Y. ; Wang, Vincent M. ; Basta-Pljakic, Jelena ; Leong, Daniel J. ; Sereysky, Jedd B. ; Ros, Stephen J. ; Klug, Raymond A. ; Braman, Jonathan ; Schaffler, Mitch B. ; Jepsen, Karl J. ; Flatow, Evan L. / Cycle-dependent matrix remodeling gene expression response in fatigue-loaded rat patellar tendons. In: Journal of Orthopaedic Research. 2010 ; Vol. 28, No. 10. pp. 1380-1386.
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AU - Sun, Hui (Herb)

AU - Andarawis-Puri, Nelly

AU - Li, Yonghui

AU - Fung, David T.

AU - Lee, Jonathan Y.

AU - Wang, Vincent M.

AU - Basta-Pljakic, Jelena

AU - Leong, Daniel J.

AU - Sereysky, Jedd B.

AU - Ros, Stephen J.

AU - Klug, Raymond A.

AU - Braman, Jonathan

AU - Schaffler, Mitch B.

AU - Jepsen, Karl J.

AU - Flatow, Evan L.

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N2 - Expression profiling of selected matrix remodeling genes was conducted to evaluate differences in molecular response to lowcycle (100) and high-cycle (7,200) sub-failure-fatigue loading of patellar tendons. Using our previously developed in vivo patellar tendon model, tendons were loaded for 100 or 7,200 cycles and expression of selected metalloproteinases (MMPs), tissue inhibitors of metalloproteinases (TIMPs), and collagens were quantified by real-time RT-PCR at 1- and 7-day post-loading. Expression profiles were also obtained from lacerated tendons as an acute injury model. The high-cycle group showed upregulation of TIMP-1, -2, Col3a1, and Col5a1, and downregulation TIMP-4 at both time points, upregulation of MMP-2 at 7-day post-loading and downregulation of MMP-13 and -14 at 1-day post-loading, suggesting overall repair/remodeling. In contrast, the low-cycle loaded group showed upregulation of MMP-2, -3, -13, and Col12a1 at both time points, upregulation of TIMP-1, -2, -3, Col3a1, and integrin b1 and downregulation of integrin a11 at 1-day post-loading and upregulation of Col1a1 at 7-day post-loading, consistent with a hypertrophic (adaptive) pattern. Lacerated tendons showed a typical acute wound response with upregulation of all examined remodeling genes. Differences found in tendon response to high- and low-cycle loading are suggestive of the underlying mechanisms associated with a healthy or damaging response.

AB - Expression profiling of selected matrix remodeling genes was conducted to evaluate differences in molecular response to lowcycle (100) and high-cycle (7,200) sub-failure-fatigue loading of patellar tendons. Using our previously developed in vivo patellar tendon model, tendons were loaded for 100 or 7,200 cycles and expression of selected metalloproteinases (MMPs), tissue inhibitors of metalloproteinases (TIMPs), and collagens were quantified by real-time RT-PCR at 1- and 7-day post-loading. Expression profiles were also obtained from lacerated tendons as an acute injury model. The high-cycle group showed upregulation of TIMP-1, -2, Col3a1, and Col5a1, and downregulation TIMP-4 at both time points, upregulation of MMP-2 at 7-day post-loading and downregulation of MMP-13 and -14 at 1-day post-loading, suggesting overall repair/remodeling. In contrast, the low-cycle loaded group showed upregulation of MMP-2, -3, -13, and Col12a1 at both time points, upregulation of TIMP-1, -2, -3, Col3a1, and integrin b1 and downregulation of integrin a11 at 1-day post-loading and upregulation of Col1a1 at 7-day post-loading, consistent with a hypertrophic (adaptive) pattern. Lacerated tendons showed a typical acute wound response with upregulation of all examined remodeling genes. Differences found in tendon response to high- and low-cycle loading are suggestive of the underlying mechanisms associated with a healthy or damaging response.

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