Cultured astrocytes express mRNA for peptidylglycine-α-amidating monooxygenase, a neuropeptide processing enzyme

Robyn S. Klein, Lloyd D. Fricker

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Cultured astrocytes have been previously found to express several neuropeptides, as well as the neuropeptide processing enzyme carboxypeptidase E (CPE). To investigate whether cultured astrocytes contain additional peptide-processing enzymes, Northern blots were screened for peptidylglycine-α-amidating monooxygenase (PAM) mRNA. PAM is involved with the formation of amide groups on the C-terminus of numerous peptide hormones and neurotransmitters. Primary cultures of astrocytes contain moderate levels of PAM mRNA, as determined by Northern blot analysis. The level of PAM mRNA is cultured hypothalamic astrocytes is similar to the level expressed in cultured hypothalamic neurons. The relative abundance of PAM mRNA differs up to 6-fold between astrocytes cultured from various brain regions. Astrocytes cultured from hypothalamus have high levels of PAM mRNA, those cultured from striatum, frontal cortex, and hippocampus have moderate levels, and those cultured from cerebellum have low levels. To investigate whether all cultured astrocytes express PAM mRNA, in situ hybridization analysis of cultured astrocytes was performed. Interestingly, virtually all of the astrocytes cultured from either hypothalamus or cerebellum express PAM mRNA, in contrast to a previous finding that only 20-40% of similarly cultured astrocytes express CPE. The presence of PAM mRNA in cultured astrocytes suggests that these cells have the capacity to produce amidated neuropeptides.

Original languageEnglish (US)
Pages (from-to)202-208
Number of pages7
JournalBrain Research
Volume596
Issue number1-2
DOIs
StatePublished - Nov 20 1992

Fingerprint

Neuropeptides
Astrocytes
Messenger RNA
Enzymes
Carboxypeptidase H
Northern Blotting
Cerebellum
Hypothalamus
peptidylglycine monooxygenase
Peptide Hormones
Frontal Lobe
Amides
In Situ Hybridization
Neurotransmitter Agents
Hippocampus
Neurons
Peptides

Keywords

  • Neuroglia
  • Neuropeptide biosynthesis
  • Prohormone processing

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Cultured astrocytes express mRNA for peptidylglycine-α-amidating monooxygenase, a neuropeptide processing enzyme. / Klein, Robyn S.; Fricker, Lloyd D.

In: Brain Research, Vol. 596, No. 1-2, 20.11.1992, p. 202-208.

Research output: Contribution to journalArticle

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