Crystallographic characterization of a Cu,Zn superoxide dismutase from Photobacterium leiognathi

Susan M. Redford, Duncan E. McRee, Elizabeth D. Getzoff, Howard M. Steinman, John A. Tainer

Research output: Contribution to journalArticle

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Abstract

Crystals of a copper-zinc Superoxide dismutase from Photobacterium leiognathi, a luminescent marine bacterium that is the species-specific symbiont of the ponyfish, have been obtained from 2-methyl-2,4-pentanediol solutions. The space group was determined using screenless small-angle precession photographs, and was confirmed by analyzing area detector diffraction data with the XENGEN programs for indexing and refinement. The crystals are monoclinic, space group C2 (a = 126.4 A ̊, b = 87.0 A ̊, c = 44.4 A ̊, β = 92.8 Å), and have two 32,000 Mr dimers per asymmetric unit. The crystals diffract to at least 2.7 Å resolution, are resistant to radiation damage, and are suitable for determination of the structure by X-ray diffraction.

Original languageEnglish (US)
Pages (from-to)449-451
Number of pages3
JournalJournal of Molecular Biology
Volume212
Issue number3
DOIs
Publication statusPublished - Apr 5 1990

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ASJC Scopus subject areas

  • Structural Biology
  • Molecular Biology

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