Crystallographic characterization of a Cu,Zn superoxide dismutase from Photobacterium leiognathi

Susan M. Redford, Duncan E. McRee, Elizabeth D. Getzoff, Howard M. Steinman, John A. Tainer

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Crystals of a copper-zinc Superoxide dismutase from Photobacterium leiognathi, a luminescent marine bacterium that is the species-specific symbiont of the ponyfish, have been obtained from 2-methyl-2,4-pentanediol solutions. The space group was determined using screenless small-angle precession photographs, and was confirmed by analyzing area detector diffraction data with the XENGEN programs for indexing and refinement. The crystals are monoclinic, space group C2 (a = 126.4 A ̊, b = 87.0 A ̊, c = 44.4 A ̊, β = 92.8 Å), and have two 32,000 Mr dimers per asymmetric unit. The crystals diffract to at least 2.7 Å resolution, are resistant to radiation damage, and are suitable for determination of the structure by X-ray diffraction.

Original languageEnglish (US)
Pages (from-to)449-451
Number of pages3
JournalJournal of Molecular Biology
Volume212
Issue number3
DOIs
StatePublished - Apr 5 1990

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Photobacterium
X-Ray Diffraction
Superoxide Dismutase
Zinc
Copper
Radiation
Bacteria
hexylene glycol
Superoxide Dismutase-1

ASJC Scopus subject areas

  • Virology

Cite this

Crystallographic characterization of a Cu,Zn superoxide dismutase from Photobacterium leiognathi. / Redford, Susan M.; McRee, Duncan E.; Getzoff, Elizabeth D.; Steinman, Howard M.; Tainer, John A.

In: Journal of Molecular Biology, Vol. 212, No. 3, 05.04.1990, p. 449-451.

Research output: Contribution to journalArticle

Redford, Susan M. ; McRee, Duncan E. ; Getzoff, Elizabeth D. ; Steinman, Howard M. ; Tainer, John A. / Crystallographic characterization of a Cu,Zn superoxide dismutase from Photobacterium leiognathi. In: Journal of Molecular Biology. 1990 ; Vol. 212, No. 3. pp. 449-451.
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