TY - JOUR
T1 - Crystallographic characterization of a Cu,Zn superoxide dismutase from Photobacterium leiognathi
AU - Redford, Susan M.
AU - McRee, Duncan E.
AU - Getzoff, Elizabeth D.
AU - Steinman, Howard M.
AU - Tainer, John A.
N1 - Funding Information:
This research was funded by National Institutes of Health grant ROl GM37684 (E.D.G. and J.A.T.) and NSF grant DMB 86-16387 (H.M.S.). D.E.M. was supported by a Helen Hay Whitney Fellowship.
PY - 1990/4/5
Y1 - 1990/4/5
N2 - Crystals of a copper-zinc Superoxide dismutase from Photobacterium leiognathi, a luminescent marine bacterium that is the species-specific symbiont of the ponyfish, have been obtained from 2-methyl-2,4-pentanediol solutions. The space group was determined using screenless small-angle precession photographs, and was confirmed by analyzing area detector diffraction data with the XENGEN programs for indexing and refinement. The crystals are monoclinic, space group C2 (a = 126.4 A ̊, b = 87.0 A ̊, c = 44.4 A ̊, β = 92.8 Å), and have two 32,000 Mr dimers per asymmetric unit. The crystals diffract to at least 2.7 Å resolution, are resistant to radiation damage, and are suitable for determination of the structure by X-ray diffraction.
AB - Crystals of a copper-zinc Superoxide dismutase from Photobacterium leiognathi, a luminescent marine bacterium that is the species-specific symbiont of the ponyfish, have been obtained from 2-methyl-2,4-pentanediol solutions. The space group was determined using screenless small-angle precession photographs, and was confirmed by analyzing area detector diffraction data with the XENGEN programs for indexing and refinement. The crystals are monoclinic, space group C2 (a = 126.4 A ̊, b = 87.0 A ̊, c = 44.4 A ̊, β = 92.8 Å), and have two 32,000 Mr dimers per asymmetric unit. The crystals diffract to at least 2.7 Å resolution, are resistant to radiation damage, and are suitable for determination of the structure by X-ray diffraction.
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U2 - 10.1016/0022-2836(90)90323-E
DO - 10.1016/0022-2836(90)90323-E
M3 - Article
C2 - 2325128
AN - SCOPUS:0025357061
SN - 0022-2836
VL - 212
SP - 449
EP - 451
JO - Journal of Molecular Biology
JF - Journal of Molecular Biology
IS - 3
ER -