Crystallization and preliminary X-ray diffraction studies of a Bowman-Birk inhibitor from Vigna unguiculata seeds

K. N. Rao, S. S. Hegde, R. J. Lewis, C. G. Suresh

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Abstract

A Bowman-Birk type trypsin/chymotrypsin inhibitor isolated from Vigna unguiculata seeds has been crystallized. Crystals were grown using the vapour-diffusion method at pH 4.0 using citrate/phosphate as a buffer and 30% saturated ammonium sulfate as precipitant. The crystals belonged to the monoclinic space group P21, with unit-cell parameters a = 32.4, b = 61.8, c = 32.9 Å, β = 114.5°. The Matthews coefficient calculated assuming two molecules in the asymmetric unit was 1.95 Å3 Da-1, which corresponds to a 37% solvent content. X-ray data were collected to 2.5 Å resolution from a flash-frozen crystal. The structure was solved using the molecular-replacement method using tracy soybean inhibitor structure (PDB entry 1pi2) as a model.

Original languageEnglish (US)
Pages (from-to)1920-1922
Number of pages3
JournalActa Crystallographica Section D: Biological Crystallography
Volume55
Issue number11
DOIs
Publication statusPublished - Nov 1 1999

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ASJC Scopus subject areas

  • Structural Biology

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