Crystal structure of a phosphorylation-coupled saccharide transporter

Yu Cao, Xiangshu Jin, Elena J. Levin, Hua Huang, Yinong Zong, Matthias Quick, Jun Weng, Yaping Pan, James Love, Marco Punta, Burkhard Rost, Wayne A. Hendrickson, Jonathan A. Javitch, Kanagalaghatta R. Rajashankar, Ming Zhou

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Saccharides have a central role in the nutrition of all living organisms. Whereas several saccharide uptake systems are shared between the different phylogenetic kingdoms, the phosphoenolpyruvate-dependent phosphotransferase system exists almost exclusively in bacteria. This multi-component system includes an integral membrane protein EIIC that transports saccharides and assists in their phosphorylation. Here we present the crystal structure of an EIIC from Bacillus cereus that transports diacetylchitobiose. The EIIC is a homodimer, with an expansive interface formed between the amino-terminal halves of the two protomers. The carboxy-terminal half of each protomer has a large binding pocket that contains a diacetylchitobiose, which is occluded from both sides of the membrane with its site of phosphorylation near the conserved His-‰250 and Glu-‰334 residues. The structure shows the architecture of this important class of transporters, identifies the determinants of substrate binding and phosphorylation, and provides a framework for understanding the mechanism of sugar translocation.

Original languageEnglish (US)
Pages (from-to)50-54
Number of pages5
JournalNature
Volume473
Issue number7345
DOIs
StatePublished - May 5 2011
Externally publishedYes

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Phosphorylation
Protein Subunits
Phosphoenolpyruvate
Bacillus cereus
Protein Transport
Membrane Proteins
Phosphotransferases
Bacteria
Membranes

ASJC Scopus subject areas

  • General

Cite this

Cao, Y., Jin, X., Levin, E. J., Huang, H., Zong, Y., Quick, M., ... Zhou, M. (2011). Crystal structure of a phosphorylation-coupled saccharide transporter. Nature, 473(7345), 50-54. https://doi.org/10.1038/nature09939

Crystal structure of a phosphorylation-coupled saccharide transporter. / Cao, Yu; Jin, Xiangshu; Levin, Elena J.; Huang, Hua; Zong, Yinong; Quick, Matthias; Weng, Jun; Pan, Yaping; Love, James; Punta, Marco; Rost, Burkhard; Hendrickson, Wayne A.; Javitch, Jonathan A.; Rajashankar, Kanagalaghatta R.; Zhou, Ming.

In: Nature, Vol. 473, No. 7345, 05.05.2011, p. 50-54.

Research output: Contribution to journalArticle

Cao, Y, Jin, X, Levin, EJ, Huang, H, Zong, Y, Quick, M, Weng, J, Pan, Y, Love, J, Punta, M, Rost, B, Hendrickson, WA, Javitch, JA, Rajashankar, KR & Zhou, M 2011, 'Crystal structure of a phosphorylation-coupled saccharide transporter', Nature, vol. 473, no. 7345, pp. 50-54. https://doi.org/10.1038/nature09939
Cao Y, Jin X, Levin EJ, Huang H, Zong Y, Quick M et al. Crystal structure of a phosphorylation-coupled saccharide transporter. Nature. 2011 May 5;473(7345):50-54. https://doi.org/10.1038/nature09939
Cao, Yu ; Jin, Xiangshu ; Levin, Elena J. ; Huang, Hua ; Zong, Yinong ; Quick, Matthias ; Weng, Jun ; Pan, Yaping ; Love, James ; Punta, Marco ; Rost, Burkhard ; Hendrickson, Wayne A. ; Javitch, Jonathan A. ; Rajashankar, Kanagalaghatta R. ; Zhou, Ming. / Crystal structure of a phosphorylation-coupled saccharide transporter. In: Nature. 2011 ; Vol. 473, No. 7345. pp. 50-54.
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