Cortactin regulates cofilin and N-WASp activities to control the stages of invadopodium assembly and maturation

Matthew Oser, Hideki Yamaguchi, Christopher C. Mader, J. J. Bravo-Cordero, Marianela Arias, Xiaoming Chen, Vera M. Desmarais, Jacco Van Rheenen, Anthony J. Koleske, John S. Condeelis

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Abstract

Invadopodia are matrix-degrading membrane protrusions in invasive carcinoma cells. The mechanisms regulating invadopodium assembly and maturation are not understood. We have dissected the stages of invadopodium assembly and maturation and show that invadopodia use cortactin phosphorylation as a master switch during these processes. In particular, cortactin phosphorylation was found to regulate cofilin and Arp2/3 complex-dependent actin polymerization. Cortactin directly binds cofilin and inhibits its severing activity. Cortactin phosphorylation is required to release this inhibition so cofilin can sever actin filaments to create barbed ends at invadopodia to support Arp2/3-dependent actin polymerization. After barbed end formation, cortactin is dephosphorylated, which blocks cofilin severing activity thereby stabilizing invadopodia. These findings identify novel mechanisms for actin polymerization in the invadopodia of metastatic carcinoma cells and define four distinct stages of invadopodium assembly and maturation consisting of invadopodium precursor formation, actin polymerization, stabilization, and matrix degradation.

Original languageEnglish (US)
Pages (from-to)571-587
Number of pages17
JournalJournal of Cell Biology
Volume186
Issue number4
DOIs
StatePublished - Aug 24 2009

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Cortactin
Actin Depolymerizing Factors
Polymerization
Actins
Phosphorylation
Actin-Related Protein 2-3 Complex
Carcinoma
Podosomes
Actin Cytoskeleton

ASJC Scopus subject areas

  • Cell Biology

Cite this

Cortactin regulates cofilin and N-WASp activities to control the stages of invadopodium assembly and maturation. / Oser, Matthew; Yamaguchi, Hideki; Mader, Christopher C.; Bravo-Cordero, J. J.; Arias, Marianela; Chen, Xiaoming; Desmarais, Vera M.; Van Rheenen, Jacco; Koleske, Anthony J.; Condeelis, John S.

In: Journal of Cell Biology, Vol. 186, No. 4, 24.08.2009, p. 571-587.

Research output: Contribution to journalArticle

Oser, M, Yamaguchi, H, Mader, CC, Bravo-Cordero, JJ, Arias, M, Chen, X, Desmarais, VM, Van Rheenen, J, Koleske, AJ & Condeelis, JS 2009, 'Cortactin regulates cofilin and N-WASp activities to control the stages of invadopodium assembly and maturation', Journal of Cell Biology, vol. 186, no. 4, pp. 571-587. https://doi.org/10.1083/jcb.200812176
Oser, Matthew ; Yamaguchi, Hideki ; Mader, Christopher C. ; Bravo-Cordero, J. J. ; Arias, Marianela ; Chen, Xiaoming ; Desmarais, Vera M. ; Van Rheenen, Jacco ; Koleske, Anthony J. ; Condeelis, John S. / Cortactin regulates cofilin and N-WASp activities to control the stages of invadopodium assembly and maturation. In: Journal of Cell Biology. 2009 ; Vol. 186, No. 4. pp. 571-587.
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