Correlation of p53 mutations in ThinPrep-processed fine needle breast aspirates with surgically resected breast cancers

Aaron Pollett, Yvan C. Bédard, Shu Qiu Li, Thomas E. Rohan, Rita Kandel

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Mutations of the p53 gene are one of the most common genetic changes found in cancer; their presence may be prognostic and even influence treatment for breast cancer. In this study, we investigated whether DNA could be extracted from the residual cells left in ThinPrep-processed breast fine-needle aspirates and whether p53 gene changes could be detected in the DNA. The results were then correlated with DNA extracted from the matched formalin-fixed, paraffin-embedded, surgically resected breast cancer when available. DNA was successfully extracted from 54 of 62 aspirates and all 31 surgical specimens. p53 gene mutations were detected in 10 of the 54 cytology specimens (18.5%) and consisted of base pair substitutions or deletions. Silent or intronic p53 changes were found in five additional aspirates. One of the aspirates had two gene alterations, resulting in a total of six gene changes. Five of these changes were located in introns 6 or 9 and the sixth was a silent (no amino acid change) change in exon 6. p53 Polymorphisms were detected in nine aspirates (16.3%) and were located in codon 47 (one aspirate), codon 72 (six aspirates), and codon 213 (two aspirates). All cases with surgical material available showed identical p53 mutations, alterations, and polymorphisms in the resected tumors compared with those detected in the corresponding aspirates. The results of this study show that DNA suitable for analysis of p53 gene sequence changes can be successfully extracted from ThinPrep-processed breast fine-needle aspirates, and that identical alterations are detected in both the cytology and surgical specimens.

Original languageEnglish (US)
Pages (from-to)1173-1179
Number of pages7
JournalModern Pathology
Volume13
Issue number11
StatePublished - 2000

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Needles
p53 Genes
Breast
Breast Neoplasms
Mutation
Codon
DNA
Cell Biology
Base Pairing
Paraffin
Introns
Formaldehyde
Genes
Exons
Neoplasms
Amino Acids
Therapeutics

Keywords

  • Breast cancer
  • Fine needle aspiration
  • P53 mutation
  • ThinPrep

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Correlation of p53 mutations in ThinPrep-processed fine needle breast aspirates with surgically resected breast cancers. / Pollett, Aaron; Bédard, Yvan C.; Li, Shu Qiu; Rohan, Thomas E.; Kandel, Rita.

In: Modern Pathology, Vol. 13, No. 11, 2000, p. 1173-1179.

Research output: Contribution to journalArticle

Pollett, Aaron ; Bédard, Yvan C. ; Li, Shu Qiu ; Rohan, Thomas E. ; Kandel, Rita. / Correlation of p53 mutations in ThinPrep-processed fine needle breast aspirates with surgically resected breast cancers. In: Modern Pathology. 2000 ; Vol. 13, No. 11. pp. 1173-1179.
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