Correlation between Protein Stability Cores and Protein Folding Kinetics

A Case Study on Pseudomonas aeruginosa Apo-Azurin

Mingzhi Chen, Corey J. Wilson, Yinghao Wu, Pernilla Wittung-Stafshede, Jianpeng Ma

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

This paper reports a combined computational and experimental study of the correlation between protein stability cores and folding kinetics. An empirical potential function was developed, and it was used for analyzing interaction energies among secondary structure elements. Studies on a β sandwich protein, Pseudomonas aeruginosa azurin, showed that the computationally identified substructure with the strongest interactions in the native state is identical to the "interlocked pair" of β strands, an invariant motif found in most sandwich-like proteins. Moreover, previous and new in vitro folding results revealed that the identified substructure harbors most residues that form native-like interactions in the folding transition state. These observations demonstrate that the potential function is effective in revealing the relative strength of interactions among various protein parts; they also strengthen the suggestion that the most stable regions in native proteins favor stable interactions early during folding.

Original languageEnglish (US)
Pages (from-to)1401-1410
Number of pages10
JournalStructure
Volume14
Issue number9
DOIs
StatePublished - Sep 2006
Externally publishedYes

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Azurin
Protein Stability
Protein Folding
Pseudomonas aeruginosa
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Structural Biology

Cite this

Correlation between Protein Stability Cores and Protein Folding Kinetics : A Case Study on Pseudomonas aeruginosa Apo-Azurin. / Chen, Mingzhi; Wilson, Corey J.; Wu, Yinghao; Wittung-Stafshede, Pernilla; Ma, Jianpeng.

In: Structure, Vol. 14, No. 9, 09.2006, p. 1401-1410.

Research output: Contribution to journalArticle

Chen, Mingzhi ; Wilson, Corey J. ; Wu, Yinghao ; Wittung-Stafshede, Pernilla ; Ma, Jianpeng. / Correlation between Protein Stability Cores and Protein Folding Kinetics : A Case Study on Pseudomonas aeruginosa Apo-Azurin. In: Structure. 2006 ; Vol. 14, No. 9. pp. 1401-1410.
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