Correlated positioning of homologous chromosomes in daughter fibroblast cells

Hui Bin Sun, Hiroki Yokota

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

A new method of analyzing chromosome segregation in pairs of daughter human fibroblasts revealed that the positioning of chromosomes in daughter nuclei was closely correlated with their relative positions during the G1 interphase. Two topographic values, namely distance and the angular separation between a pair of homologous chromosomes, were determined using fluorescence in-situ hybridization with four different centromeric DNA probes. These topographical values exhibited a broad distribution as a population, but, to our surprise, both were strongly correlated within each pair of cells derived from the same mother cell (daughters). This correlation was not affected by cell-to-cell distances between daughter cells. We demonstrate in this report that the positioning of chromosomes at G1 interphase is chiefly determined by their configuration at mitosis, consistent with the nuclear architecture model in which chromosomes are immobile at a global scale in the G1 interphase nuclei.

Original languageEnglish (US)
Pages (from-to)603-610
Number of pages8
JournalChromosome Research
Volume7
Issue number8
DOIs
Publication statusPublished - Dec 1 1999
Externally publishedYes

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Keywords

  • Chromosome positioning
  • Daughter cells
  • Fluoresence in-situ hybridization
  • Human fibroblast
  • Interphase nucleus

ASJC Scopus subject areas

  • Genetics

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