Coordinate replication of members of the multigene family of core and H1 human histone genes

M. A. Iqbal, M. Plumb, J. Stein, Carl L. Schildkraut

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Cells of the K562 human erythroleukemia cell line were obtained in different stages of the cell cycle by centrifugal elutriation. The cells had been previously labeled for 2 hr with BrdUrd so that BrdUrd-DNA synthesized during four different selected intervals of the S phase could be isolated. This DNA was used to determine the temporal replication during S phase of EcoRI segments containing histone genes. Cloned human genomic segments containing the core histone genes (H2A, H2B, H3, and H4), H2A and H2B pseudogenes, and the H1 gene were prepared. The genomic inserts were excised from these plasmids, nick-translated, and used as hybridization probes. The results with different probes compared on the same and on independently prepared DBM-paper transfers indicate that all of these histone genes replicate during the first half of the S phase. These genes were not among the earliest to replicate in the K562 cell line. Similar studies were carried out with HeLa cells in which EcoRI segments containing the H4 histone and H2A and H2B pseudogenes were found to replicate during the interval of the S phase when histone mRNA appears in the cytoplasm at the maximal rate. The possible relationship between these events is discussed.

Original languageEnglish (US)
Pages (from-to)7723-7727
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume81
Issue number24 I
DOIs
StatePublished - 1984
Externally publishedYes

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Multigene Family
Histones
S Phase
Pseudogenes
K562 Cells
Genes
Cell Line
Leukemia, Erythroblastic, Acute
DNA
HeLa Cells
Cell Cycle
Cytoplasm
Plasmids
Messenger RNA

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Coordinate replication of members of the multigene family of core and H1 human histone genes. / Iqbal, M. A.; Plumb, M.; Stein, J.; Schildkraut, Carl L.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 81, No. 24 I, 1984, p. 7723-7727.

Research output: Contribution to journalArticle

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