Cooperation between a coenzyme A-independent stand-alone initiation module and an iterative type I polyketide synthase during synthesis of mycobacterial phenolic glycolipids

Weiguo He, Clifford E. Soll, Sivagami Sundaram Chavadi, Guangtao Zhang, J. David Warren, Luis E N Quadri

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Several Mycobacterium tuberculosis strains, Mycobacterium leprae, and other mycobacterial pathogens produce a group of small-molecule virulence factors called phenolic glycolipids (PGLs). PGLs play key roles in pathogenicity and host-pathogen interaction. Thus, elucidation of the PGL biosynthetic pathway will not only expand our understanding of natural product biosynthesis, but may also illuminate routes to novel therapeutics to afford alternative lines of defense against mycobacterial infections. In this study, we report an investigation of the enzymatic requirements for the production of long-chain p-hydroxyphenylalkanoate intermediates of PGL biosynthesis. We demonstrate a functional cooperation between a coenzyme A-independent stand-alone didomain initiation module (FadD22) and a 6-domain reducing iterative type I polyketide synthase (Pks15/1) for production of p-hydroxyphenylalkanoate intermediates in in vitro and in vivo FadD22-Pks15/1 reconstituted systems. Our results suggest that Pks15/1 is an iterative type I polyketide synthase with a relaxed control of catalytic cycle iterations, a mechanistic property that explains the origin of a characteristic alkyl chain length variability seen in mycobacterial PGLs. The FadD22-Pks15/1 reconstituted systems lay an initial foundation for future efforts to unveil the mechanism of iterative catalysis control by which the structures of the final products of Pks15/1 are defined, and to scrutinize the functional partnerships of the FadD22-Pks15/1 system with downstream enzymes of the PGL biosynthetic pathway.

Original languageEnglish (US)
Pages (from-to)16744-16750
Number of pages7
JournalJournal of the American Chemical Society
Volume131
Issue number46
DOIs
StatePublished - 2009
Externally publishedYes

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Polyketide Synthases
Coenzymes
Glycolipids
Coenzyme A
Biosynthesis
Biosynthetic Pathways
Pathogens
Host-Pathogen Interactions
Mycobacterium leprae
Virulence Factors
Biological Products
Catalysis
Chain length
Mycobacterium tuberculosis
Virulence
Enzymes
Molecules
Infection

ASJC Scopus subject areas

  • Chemistry(all)
  • Catalysis
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

Cooperation between a coenzyme A-independent stand-alone initiation module and an iterative type I polyketide synthase during synthesis of mycobacterial phenolic glycolipids. / He, Weiguo; Soll, Clifford E.; Chavadi, Sivagami Sundaram; Zhang, Guangtao; Warren, J. David; Quadri, Luis E N.

In: Journal of the American Chemical Society, Vol. 131, No. 46, 2009, p. 16744-16750.

Research output: Contribution to journalArticle

He, Weiguo ; Soll, Clifford E. ; Chavadi, Sivagami Sundaram ; Zhang, Guangtao ; Warren, J. David ; Quadri, Luis E N. / Cooperation between a coenzyme A-independent stand-alone initiation module and an iterative type I polyketide synthase during synthesis of mycobacterial phenolic glycolipids. In: Journal of the American Chemical Society. 2009 ; Vol. 131, No. 46. pp. 16744-16750.
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