Contamination of perfused donor kidneys by starch from surgical gloves

S. Moriber-Katz, S. Goldstein, D. Ferluga, Stuart M. Greenstein, A. S. Miller, A. B. Schwartz, S. Simonian

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Starch induces a variety of inflammatory reactions in humans and may contaminate surgical procedures from surgical gloves. Using polarized microscopy and electron microscopy, the authors observed starch particles in renal perfusate and in glomeruli of perfused donor kidneys. Group 1 consisted of 10 unusuable kidneys handled with standard concern of glove sterility. Eight other kidneys (Group 2) were perfused with particular attention toward avoidance of starch contamination. The gloves were rinsed five times, and the cuffs were not dipped into the perfusate. Two kidneys (Group 3), deemed unsuitable for transplantation, were perfused for 24 hours with perfusate swished with unwashed sterile gloves. Group 4 consisted of five transplant biopsies performed within one week after transplantation. Perfusates alone were also circulated through the Waters Perfusion Machine continuously for 24, 72, and 188 hours with and without the use of gloves. The number of birefringent crosses were counted in each of 25 glomeruli per specimen. Group 1 displayed a mean of 1.8 birefringent crosses per glomerulus; Group 2, 0; Group 3, 5.3; and Group 4, 4.4. Groups 1 and 3 also exhibited birefringent crosses in peripheral renal vessels. Perfusate alone, handled without gloves, showed no birefringent crosses; by contrast, perfusate handled with gloves showed numerous birefringent crosses. The authors conclude that starch from surgical gloves can enter the perfusate and lodge in glomeruli and other sites of donor kidneys. Rinsing gloves five times and avoiding contact of the perfusate with the glove cuff effectively eliminates the contamination.

Original languageEnglish (US)
Pages (from-to)81-84
Number of pages4
JournalAmerican Journal of Clinical Pathology
Volume90
Issue number1
StatePublished - 1988
Externally publishedYes

Fingerprint

Surgical Gloves
Starch
Kidney
Transplantation
Infertility
Microscopy
Electron Microscopy
Perfusion
Transplants
Biopsy
Water

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Moriber-Katz, S., Goldstein, S., Ferluga, D., Greenstein, S. M., Miller, A. S., Schwartz, A. B., & Simonian, S. (1988). Contamination of perfused donor kidneys by starch from surgical gloves. American Journal of Clinical Pathology, 90(1), 81-84.

Contamination of perfused donor kidneys by starch from surgical gloves. / Moriber-Katz, S.; Goldstein, S.; Ferluga, D.; Greenstein, Stuart M.; Miller, A. S.; Schwartz, A. B.; Simonian, S.

In: American Journal of Clinical Pathology, Vol. 90, No. 1, 1988, p. 81-84.

Research output: Contribution to journalArticle

Moriber-Katz, S, Goldstein, S, Ferluga, D, Greenstein, SM, Miller, AS, Schwartz, AB & Simonian, S 1988, 'Contamination of perfused donor kidneys by starch from surgical gloves', American Journal of Clinical Pathology, vol. 90, no. 1, pp. 81-84.
Moriber-Katz S, Goldstein S, Ferluga D, Greenstein SM, Miller AS, Schwartz AB et al. Contamination of perfused donor kidneys by starch from surgical gloves. American Journal of Clinical Pathology. 1988;90(1):81-84.
Moriber-Katz, S. ; Goldstein, S. ; Ferluga, D. ; Greenstein, Stuart M. ; Miller, A. S. ; Schwartz, A. B. ; Simonian, S. / Contamination of perfused donor kidneys by starch from surgical gloves. In: American Journal of Clinical Pathology. 1988 ; Vol. 90, No. 1. pp. 81-84.
@article{cf2dd02b496f4a2fb071f9c4aa6192c2,
title = "Contamination of perfused donor kidneys by starch from surgical gloves",
abstract = "Starch induces a variety of inflammatory reactions in humans and may contaminate surgical procedures from surgical gloves. Using polarized microscopy and electron microscopy, the authors observed starch particles in renal perfusate and in glomeruli of perfused donor kidneys. Group 1 consisted of 10 unusuable kidneys handled with standard concern of glove sterility. Eight other kidneys (Group 2) were perfused with particular attention toward avoidance of starch contamination. The gloves were rinsed five times, and the cuffs were not dipped into the perfusate. Two kidneys (Group 3), deemed unsuitable for transplantation, were perfused for 24 hours with perfusate swished with unwashed sterile gloves. Group 4 consisted of five transplant biopsies performed within one week after transplantation. Perfusates alone were also circulated through the Waters Perfusion Machine continuously for 24, 72, and 188 hours with and without the use of gloves. The number of birefringent crosses were counted in each of 25 glomeruli per specimen. Group 1 displayed a mean of 1.8 birefringent crosses per glomerulus; Group 2, 0; Group 3, 5.3; and Group 4, 4.4. Groups 1 and 3 also exhibited birefringent crosses in peripheral renal vessels. Perfusate alone, handled without gloves, showed no birefringent crosses; by contrast, perfusate handled with gloves showed numerous birefringent crosses. The authors conclude that starch from surgical gloves can enter the perfusate and lodge in glomeruli and other sites of donor kidneys. Rinsing gloves five times and avoiding contact of the perfusate with the glove cuff effectively eliminates the contamination.",
author = "S. Moriber-Katz and S. Goldstein and D. Ferluga and Greenstein, {Stuart M.} and Miller, {A. S.} and Schwartz, {A. B.} and S. Simonian",
year = "1988",
language = "English (US)",
volume = "90",
pages = "81--84",
journal = "American Journal of Clinical Pathology",
issn = "0002-9173",
publisher = "American Society of Clinical Pathologists",
number = "1",

}

TY - JOUR

T1 - Contamination of perfused donor kidneys by starch from surgical gloves

AU - Moriber-Katz, S.

AU - Goldstein, S.

AU - Ferluga, D.

AU - Greenstein, Stuart M.

AU - Miller, A. S.

AU - Schwartz, A. B.

AU - Simonian, S.

PY - 1988

Y1 - 1988

N2 - Starch induces a variety of inflammatory reactions in humans and may contaminate surgical procedures from surgical gloves. Using polarized microscopy and electron microscopy, the authors observed starch particles in renal perfusate and in glomeruli of perfused donor kidneys. Group 1 consisted of 10 unusuable kidneys handled with standard concern of glove sterility. Eight other kidneys (Group 2) were perfused with particular attention toward avoidance of starch contamination. The gloves were rinsed five times, and the cuffs were not dipped into the perfusate. Two kidneys (Group 3), deemed unsuitable for transplantation, were perfused for 24 hours with perfusate swished with unwashed sterile gloves. Group 4 consisted of five transplant biopsies performed within one week after transplantation. Perfusates alone were also circulated through the Waters Perfusion Machine continuously for 24, 72, and 188 hours with and without the use of gloves. The number of birefringent crosses were counted in each of 25 glomeruli per specimen. Group 1 displayed a mean of 1.8 birefringent crosses per glomerulus; Group 2, 0; Group 3, 5.3; and Group 4, 4.4. Groups 1 and 3 also exhibited birefringent crosses in peripheral renal vessels. Perfusate alone, handled without gloves, showed no birefringent crosses; by contrast, perfusate handled with gloves showed numerous birefringent crosses. The authors conclude that starch from surgical gloves can enter the perfusate and lodge in glomeruli and other sites of donor kidneys. Rinsing gloves five times and avoiding contact of the perfusate with the glove cuff effectively eliminates the contamination.

AB - Starch induces a variety of inflammatory reactions in humans and may contaminate surgical procedures from surgical gloves. Using polarized microscopy and electron microscopy, the authors observed starch particles in renal perfusate and in glomeruli of perfused donor kidneys. Group 1 consisted of 10 unusuable kidneys handled with standard concern of glove sterility. Eight other kidneys (Group 2) were perfused with particular attention toward avoidance of starch contamination. The gloves were rinsed five times, and the cuffs were not dipped into the perfusate. Two kidneys (Group 3), deemed unsuitable for transplantation, were perfused for 24 hours with perfusate swished with unwashed sterile gloves. Group 4 consisted of five transplant biopsies performed within one week after transplantation. Perfusates alone were also circulated through the Waters Perfusion Machine continuously for 24, 72, and 188 hours with and without the use of gloves. The number of birefringent crosses were counted in each of 25 glomeruli per specimen. Group 1 displayed a mean of 1.8 birefringent crosses per glomerulus; Group 2, 0; Group 3, 5.3; and Group 4, 4.4. Groups 1 and 3 also exhibited birefringent crosses in peripheral renal vessels. Perfusate alone, handled without gloves, showed no birefringent crosses; by contrast, perfusate handled with gloves showed numerous birefringent crosses. The authors conclude that starch from surgical gloves can enter the perfusate and lodge in glomeruli and other sites of donor kidneys. Rinsing gloves five times and avoiding contact of the perfusate with the glove cuff effectively eliminates the contamination.

UR - http://www.scopus.com/inward/record.url?scp=0023920398&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023920398&partnerID=8YFLogxK

M3 - Article

C2 - 3291607

AN - SCOPUS:0023920398

VL - 90

SP - 81

EP - 84

JO - American Journal of Clinical Pathology

JF - American Journal of Clinical Pathology

SN - 0002-9173

IS - 1

ER -