Construction of immune library of murine immunoglobulin genes and screening of single-chain Fv-antibodies to diphtheria toxin B subunit

O. S. Oliinyk, Andrii Kaberniuk, T. A. Redchuk, N. V. Korotkevich, A. J. Labyntsev, S. I. Romanyuk, D. V. Kolibo, S. V. Komisarenko

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The aim of this work was to obtain the panel of recombinant single-chain Fv-antibodies against diphtheria toxin B subunit, the main diagnostic and pathogenic antigen of Corynebacterium diph-theriae. For this purpose we have constructed the immune library of murine immunoglobulin genes.A number of scFv specific to diphtheria toxin B subunit were acquired from the obtained library after one round of selection by phage-display. ScFv encoding DNA-fragments of eight clones were subcloned into plasmid pET-22b(+). It was shown that selected scFv were highly specific to diphtheria toxin B subunit, with affinity constant for different clones ranged from 10 7 to 10 9 M -1.

Original languageEnglish (US)
Pages (from-to)68-79
Number of pages12
JournalUkrain'skyi Biokhimichnyi Zhurnal
Volume81
Issue number2
StatePublished - 2009
Externally publishedYes

Fingerprint

Diphtheria Toxin
Single-Chain Antibodies
Immunoglobulin Genes
Libraries
Immunoglobulins
Screening
Genes
Clone Cells
Corynebacterium
Bacteriophages
Plasmids
Display devices
Antigens
DNA

Keywords

  • Diphtheria toxin
  • Library of immunoglobulin genes
  • Phage display
  • Scfv
  • Single chain antibodies

ASJC Scopus subject areas

  • Biochemistry

Cite this

Oliinyk, O. S., Kaberniuk, A., Redchuk, T. A., Korotkevich, N. V., Labyntsev, A. J., Romanyuk, S. I., ... Komisarenko, S. V. (2009). Construction of immune library of murine immunoglobulin genes and screening of single-chain Fv-antibodies to diphtheria toxin B subunit. Ukrain'skyi Biokhimichnyi Zhurnal, 81(2), 68-79.

Construction of immune library of murine immunoglobulin genes and screening of single-chain Fv-antibodies to diphtheria toxin B subunit. / Oliinyk, O. S.; Kaberniuk, Andrii; Redchuk, T. A.; Korotkevich, N. V.; Labyntsev, A. J.; Romanyuk, S. I.; Kolibo, D. V.; Komisarenko, S. V.

In: Ukrain'skyi Biokhimichnyi Zhurnal, Vol. 81, No. 2, 2009, p. 68-79.

Research output: Contribution to journalArticle

Oliinyk, OS, Kaberniuk, A, Redchuk, TA, Korotkevich, NV, Labyntsev, AJ, Romanyuk, SI, Kolibo, DV & Komisarenko, SV 2009, 'Construction of immune library of murine immunoglobulin genes and screening of single-chain Fv-antibodies to diphtheria toxin B subunit', Ukrain'skyi Biokhimichnyi Zhurnal, vol. 81, no. 2, pp. 68-79.
Oliinyk, O. S. ; Kaberniuk, Andrii ; Redchuk, T. A. ; Korotkevich, N. V. ; Labyntsev, A. J. ; Romanyuk, S. I. ; Kolibo, D. V. ; Komisarenko, S. V. / Construction of immune library of murine immunoglobulin genes and screening of single-chain Fv-antibodies to diphtheria toxin B subunit. In: Ukrain'skyi Biokhimichnyi Zhurnal. 2009 ; Vol. 81, No. 2. pp. 68-79.
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