Construction of immune library of murine immunoglobulin genes and screening of single-chain Fv-antibodies to diphtheria toxin B subunit

O. S. Oliinyk, A. A. Kaberniuk, T. A. Redchuk, N. V. Korotkevich, A. J. Labyntsev, S. I. Romanyuk, D. V. Kolibo, S. V. Komisarenko

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

The aim of this work was to obtain the panel of recombinant single-chain Fv-antibodies against diphtheria toxin B subunit, the main diagnostic and pathogenic antigen of Corynebacterium diph-theriae. For this purpose we have constructed the immune library of murine immunoglobulin genes.A number of scFv specific to diphtheria toxin B subunit were acquired from the obtained library after one round of selection by phage-display. ScFv encoding DNA-fragments of eight clones were subcloned into plasmid pET-22b(+). It was shown that selected scFv were highly specific to diphtheria toxin B subunit, with affinity constant for different clones ranged from 10 7 to 10 9 M -1.

Original languageEnglish (US)
Pages (from-to)68-79
Number of pages12
JournalUkrain'skyi Biokhimichnyi Zhurnal
Volume81
Issue number2
StatePublished - Jul 7 2009

Keywords

  • Diphtheria toxin
  • Library of immunoglobulin genes
  • Phage display
  • Scfv
  • Single chain antibodies

ASJC Scopus subject areas

  • Biochemistry

Fingerprint Dive into the research topics of 'Construction of immune library of murine immunoglobulin genes and screening of single-chain Fv-antibodies to diphtheria toxin B subunit'. Together they form a unique fingerprint.

  • Cite this

    Oliinyk, O. S., Kaberniuk, A. A., Redchuk, T. A., Korotkevich, N. V., Labyntsev, A. J., Romanyuk, S. I., Kolibo, D. V., & Komisarenko, S. V. (2009). Construction of immune library of murine immunoglobulin genes and screening of single-chain Fv-antibodies to diphtheria toxin B subunit. Ukrain'skyi Biokhimichnyi Zhurnal, 81(2), 68-79.