Construction of an Escherichia coli K-12 strain deleted for manganese and iron superoxide dismutase genes and its use in cloning the iron superoxide dismutase gene of Legionella pneumophila

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Abstract

An Escherichia coli K-12 strain deleted for sodA and sodB (manganese and iron superoxide dismulases) was constructed and characterized by Southern blotting, enzyme assays, and physiological analyses. The sod deletion strain was used to clone the iron superoxide dismutase gene of Legionella pneumophila by complementation to paraquat resistance.

Original languageEnglish (US)
Pages (from-to)427-430
Number of pages4
JournalMGG Molecular & General Genetics
Volume232
Issue number3
DOIs
StatePublished - Apr 1992

Fingerprint

Legionella pneumophila
Paraquat
Enzyme Assays
Manganese
Southern Blotting
Superoxides
Superoxide Dismutase
Organism Cloning
Iron
Clone Cells
Escherichia coli
Genes

Keywords

  • Deletion mutants
  • Escherichia coli
  • Legionella pneumophila
  • Superoxide dismutase

ASJC Scopus subject areas

  • Genetics

Cite this

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abstract = "An Escherichia coli K-12 strain deleted for sodA and sodB (manganese and iron superoxide dismulases) was constructed and characterized by Southern blotting, enzyme assays, and physiological analyses. The sod deletion strain was used to clone the iron superoxide dismutase gene of Legionella pneumophila by complementation to paraquat resistance.",
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AB - An Escherichia coli K-12 strain deleted for sodA and sodB (manganese and iron superoxide dismulases) was constructed and characterized by Southern blotting, enzyme assays, and physiological analyses. The sod deletion strain was used to clone the iron superoxide dismutase gene of Legionella pneumophila by complementation to paraquat resistance.

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KW - Superoxide dismutase

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