Construction of an Escherichia coli K-12 strain deleted for manganese and iron superoxide dismutase genes and its use in cloning the iron superoxide dismutase gene of Legionella pneumophila

Research output: Contribution to journalArticle

31 Scopus citations

Abstract

An Escherichia coli K-12 strain deleted for sodA and sodB (manganese and iron superoxide dismulases) was constructed and characterized by Southern blotting, enzyme assays, and physiological analyses. The sod deletion strain was used to clone the iron superoxide dismutase gene of Legionella pneumophila by complementation to paraquat resistance.

Original languageEnglish (US)
Pages (from-to)427-430
Number of pages4
JournalMGG Molecular & General Genetics
Volume232
Issue number3
DOIs
StatePublished - Apr 1 1992

Keywords

  • Deletion mutants
  • Escherichia coli
  • Legionella pneumophila
  • Superoxide dismutase

ASJC Scopus subject areas

  • Genetics

Fingerprint Dive into the research topics of 'Construction of an Escherichia coli K-12 strain deleted for manganese and iron superoxide dismutase genes and its use in cloning the iron superoxide dismutase gene of Legionella pneumophila'. Together they form a unique fingerprint.

  • Cite this