Abstract
The modification of cardiovascular stent surface for a better micro-environment has gradually changed to multi-molecule, multi-functional designation. In this study, heparin (Hep) and type IV collagen (IVCol) were used as the functional molecule to construct a bifunctional micro-environment of anticoagulation and promoting endothelialization on titanium (Ti). The surface characterization results (AFM, Alcian Blue 8GX Staining and fluorescence staining of IVCol) indicated that the bio-layer of Hep and IVCol were successfully fabricated on the Ti surface through electrostatic self-assembly. The APTT and platelet adhesion test demonstrated that the bionic layer possessed better blood compatibility compared with Ti surface. The adhesion, proliferation, migration and apoptosis tests of endothelial cells proved that the Hep/IVCol layer was able to enhance the endothelialization of the Ti surface. The in vivo animal implantation results manifested that the bionic surface could encourage new endothelialization. This work provides an important reference for the construction of multifunction micro-environment on the cardiovascular scaffold surface.
| Original language | English (US) |
|---|---|
| Article number | 81 |
| Journal | Journal of Materials Science: Materials in Medicine |
| Volume | 27 |
| Issue number | 4 |
| DOIs | |
| State | Published - Apr 1 2016 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Bioengineering
- Biophysics
- Biomaterials
- Biomedical Engineering
Cite this
Constructing bio-layer of heparin and type IV collagen on titanium surface for improving its endothelialization and blood compatibility. / Zhang, Kun; Chen, Jun ying; Qin, Wei; Li, Jing an; Guan, Fangxia; Huang, Nan.
In: Journal of Materials Science: Materials in Medicine, Vol. 27, No. 4, 81, 01.04.2016.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Constructing bio-layer of heparin and type IV collagen on titanium surface for improving its endothelialization and blood compatibility
AU - Zhang, Kun
AU - Chen, Jun ying
AU - Qin, Wei
AU - Li, Jing an
AU - Guan, Fangxia
AU - Huang, Nan
PY - 2016/4/1
Y1 - 2016/4/1
N2 - The modification of cardiovascular stent surface for a better micro-environment has gradually changed to multi-molecule, multi-functional designation. In this study, heparin (Hep) and type IV collagen (IVCol) were used as the functional molecule to construct a bifunctional micro-environment of anticoagulation and promoting endothelialization on titanium (Ti). The surface characterization results (AFM, Alcian Blue 8GX Staining and fluorescence staining of IVCol) indicated that the bio-layer of Hep and IVCol were successfully fabricated on the Ti surface through electrostatic self-assembly. The APTT and platelet adhesion test demonstrated that the bionic layer possessed better blood compatibility compared with Ti surface. The adhesion, proliferation, migration and apoptosis tests of endothelial cells proved that the Hep/IVCol layer was able to enhance the endothelialization of the Ti surface. The in vivo animal implantation results manifested that the bionic surface could encourage new endothelialization. This work provides an important reference for the construction of multifunction micro-environment on the cardiovascular scaffold surface.
AB - The modification of cardiovascular stent surface for a better micro-environment has gradually changed to multi-molecule, multi-functional designation. In this study, heparin (Hep) and type IV collagen (IVCol) were used as the functional molecule to construct a bifunctional micro-environment of anticoagulation and promoting endothelialization on titanium (Ti). The surface characterization results (AFM, Alcian Blue 8GX Staining and fluorescence staining of IVCol) indicated that the bio-layer of Hep and IVCol were successfully fabricated on the Ti surface through electrostatic self-assembly. The APTT and platelet adhesion test demonstrated that the bionic layer possessed better blood compatibility compared with Ti surface. The adhesion, proliferation, migration and apoptosis tests of endothelial cells proved that the Hep/IVCol layer was able to enhance the endothelialization of the Ti surface. The in vivo animal implantation results manifested that the bionic surface could encourage new endothelialization. This work provides an important reference for the construction of multifunction micro-environment on the cardiovascular scaffold surface.
UR - http://www.scopus.com/inward/record.url?scp=84959563266&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84959563266&partnerID=8YFLogxK
U2 - 10.1007/s10856-016-5693-6
DO - 10.1007/s10856-016-5693-6
M3 - Article
C2 - 26936367
AN - SCOPUS:84959563266
VL - 27
JO - Journal of Materials Science: Materials in Medicine
JF - Journal of Materials Science: Materials in Medicine
SN - 0957-4530
IS - 4
M1 - 81
ER -