Constructing bio-layer of heparin and type IV collagen on titanium surface for improving its endothelialization and blood compatibility

Kun Zhang, Jun ying Chen, Wei Qin, Jing an Li, Fangxia Guan, Nan Huang

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The modification of cardiovascular stent surface for a better micro-environment has gradually changed to multi-molecule, multi-functional designation. In this study, heparin (Hep) and type IV collagen (IVCol) were used as the functional molecule to construct a bifunctional micro-environment of anticoagulation and promoting endothelialization on titanium (Ti). The surface characterization results (AFM, Alcian Blue 8GX Staining and fluorescence staining of IVCol) indicated that the bio-layer of Hep and IVCol were successfully fabricated on the Ti surface through electrostatic self-assembly. The APTT and platelet adhesion test demonstrated that the bionic layer possessed better blood compatibility compared with Ti surface. The adhesion, proliferation, migration and apoptosis tests of endothelial cells proved that the Hep/IVCol layer was able to enhance the endothelialization of the Ti surface. The in vivo animal implantation results manifested that the bionic surface could encourage new endothelialization. This work provides an important reference for the construction of multifunction micro-environment on the cardiovascular scaffold surface.

Original languageEnglish (US)
Article number81
JournalJournal of Materials Science: Materials in Medicine
Volume27
Issue number4
DOIs
StatePublished - Apr 1 2016
Externally publishedYes

Fingerprint

Collagen Type IV
Titanium
Collagen
Heparin
Blood
Bionics
Staining and Labeling
Alcian Blue
Static Electricity
Adhesion
Stents
Blood Platelets
Endothelial Cells
Fluorescence
Apoptosis
Molecules
Endothelial cells
Cell death
Platelets
Scaffolds

ASJC Scopus subject areas

  • Bioengineering
  • Biophysics
  • Biomaterials
  • Biomedical Engineering

Cite this

Constructing bio-layer of heparin and type IV collagen on titanium surface for improving its endothelialization and blood compatibility. / Zhang, Kun; Chen, Jun ying; Qin, Wei; Li, Jing an; Guan, Fangxia; Huang, Nan.

In: Journal of Materials Science: Materials in Medicine, Vol. 27, No. 4, 81, 01.04.2016.

Research output: Contribution to journalArticle

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