TY - JOUR
T1 - Concentration effects in the hydrodynamic properties of deoxyribonucleic acid
AU - Eigner, Joseph
AU - Schildkraut, Carl
AU - Doty, Paul
N1 - Funding Information:
We wish to thank Professor J. MARMUR for advice and aid and Dr. H. VAN VUN^KIS for th e sample of T6 DNA. We are grateful to Drs, J. HEASST, J. VINOG~D, A. D. HERSHEY AND J. B. T. ArSN for making their unpublished observations on the speed effect in sedimentation available to us. This work was supported by a grant from the National Cancer Institute (C-2170).
PY - 1962/1/22
Y1 - 1962/1/22
N2 - The influence of solute concentration on measurements of the viscosity and sedimentation properties of DNA solutions is very great. Enough data has been accumulated in recent years, however, to permit a quantitative description of these concentration effects for DNA samples of molecular weight below roughly 16·106. It has therefore become possible to measure [η] and s0 (and indirectly molecular weight) for such samples from single measurements on generally available equipment. Above molecular weight 16·106 the possibility of such a simplification is at present excluded by the magnitude of the concentration effect in sedimentation and the gradient effect in viscosity, plus the newly studied phenomenon of speed dependence in sedimentation. It is shown, however, that rotor speed does not influence the sedimentation of samples up to molecular weight 16·106 until a critical high velocity is reached.
AB - The influence of solute concentration on measurements of the viscosity and sedimentation properties of DNA solutions is very great. Enough data has been accumulated in recent years, however, to permit a quantitative description of these concentration effects for DNA samples of molecular weight below roughly 16·106. It has therefore become possible to measure [η] and s0 (and indirectly molecular weight) for such samples from single measurements on generally available equipment. Above molecular weight 16·106 the possibility of such a simplification is at present excluded by the magnitude of the concentration effect in sedimentation and the gradient effect in viscosity, plus the newly studied phenomenon of speed dependence in sedimentation. It is shown, however, that rotor speed does not influence the sedimentation of samples up to molecular weight 16·106 until a critical high velocity is reached.
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U2 - 10.1016/0006-3002(62)90926-5
DO - 10.1016/0006-3002(62)90926-5
M3 - Article
C2 - 13889615
AN - SCOPUS:50549174216
SN - 0006-3002
VL - 55
SP - 13
EP - 21
JO - Biochimica et biophysica acta
JF - Biochimica et biophysica acta
IS - 1-2
ER -