Computer Assisted Assembly of Connectomes from Electron Micrographs: Application to Caenorhabditis elegans

Meng Xu, Travis A. Jarrell, Yi Wang, Steven J. Cook, David H. Hall, Scott W. Emmons

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

A rate-limiting step in determining a connectome, the set of all synaptic connections in a nervous system, is extraction of the relevant information from serial electron micrographs. Here we introduce a software application, Elegance, that speeds acquisition of the minimal dataset necessary, allowing the discovery of new connectomes. We have used Elegance to obtain new connectivity data in the nematode worm Caenorhabditis elegans. We analyze the accuracy that can be obtained, which is limited by unresolvable ambiguities at some locations in electron microscopic images. Elegance is useful for reconstructing connectivity in any region of neuropil of sufficiently small size.

Original languageEnglish (US)
Article numbere54050
JournalPLoS One
Volume8
Issue number1
DOIs
StatePublished - Jan 16 2013

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Connectome
Caenorhabditis elegans
electrons
Electrons
Neuropil
Information Storage and Retrieval
Neurology
Application programs
nervous system
Nervous System
Software
Nematoda

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Computer Assisted Assembly of Connectomes from Electron Micrographs : Application to Caenorhabditis elegans. / Xu, Meng; Jarrell, Travis A.; Wang, Yi; Cook, Steven J.; Hall, David H.; Emmons, Scott W.

In: PLoS One, Vol. 8, No. 1, e54050, 16.01.2013.

Research output: Contribution to journalArticle

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