Computational methods for analysis of two-dimensional gels.

Gorka Lasso, Rune Matthiesen

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Two-dimensional gel electrophoresis (2D gels) is an essential quantitative proteomics technique that is frequently used to study differences between samples of clinical relevance. Although considered to have a low throughput, 2D gels can separate thousands of proteins in one gel, making it a good complementary method to MS-based protein quantification. The main drawback of the technique is the tendency of large and hydrophobic proteins such as membrane proteins to precipitate in the isoelectric focusing step. Furthermore, tests using different programs with distinct algorithms for 2D-gel analysis have shown inconsistent ratio values. The aim here is therefore to provide a discussion of algorithms described for the analysis of 2D gels.

Original languageEnglish (US)
Pages (from-to)231-262
Number of pages32
JournalMethods in molecular biology (Clifton, N.J.)
Volume593
StatePublished - 2010
Externally publishedYes

Fingerprint

Gels
Proteins
Electrophoresis, Gel, Two-Dimensional
Isoelectric Focusing
Proteomics
Membrane Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Computational methods for analysis of two-dimensional gels. / Lasso, Gorka; Matthiesen, Rune.

In: Methods in molecular biology (Clifton, N.J.), Vol. 593, 2010, p. 231-262.

Research output: Contribution to journalArticle

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